476 Result(s)
within
Protocol
Lu**g Li
Biomedicine
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Protocol
Orthotopic Model of Hepatocellular Carcinoma in Mice
Orthotopic models of hepatocellular carcinoma (HCC) consist in the implantation of tumor cells into the liver by direct intrahepatic injection. In this model, tumorigenesis is triggered within the hepatic micr...
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Protocol
Two-Photon FRET/FLIM Imaging of Cerebral Neurons
Two-photon FRET (Förster resonance energy transfer) and FLIM (fluorescence lifetime imaging microscopy) enable the detection of FRET changes of fluorescence reporters in deep brain tissues, which provide a val...
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Protocol
A Mouse Model of Hepatocellular Carcinoma Induced by Streptozotocin and High-Fat Diet
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and the second most common cause of cancer-related death. HCC is associated to chronic diseases such as viral hepatitis, alcoholic, and no...
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Targeted Analysis of Glycerophospholipids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer
The metabolic rearrangements of hepatic metabolism associated with liver cancer are still incompletely understood. There is an ongoing need to identify novel and more efficient diagnostic biomarkers and therap...
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Protocol
A Robust In Vitro Culture Model and Generation of Memory Monocytes
Innate monocytes can be trained or reprogrammed to adopt distinct memory states, such as low-grade inflammation and immune exhaustion, bearing fundamental relevance to the pathogenesis of both acute diseases s...
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Protocol
Deletion of Large Intergenic Chromosomal Fragments in Human Induced Pluripotent Stem Cells (hiPSC) by CRISPR/Cas9 Genome Editing
Genome-wide map** interactions between the proximal/distal enhancers and gene promoters display landscapes of the spatiotemporal gene expression in the context of tissue complexity. Increasing data demonstra...
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Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye
Infection by positive-strand RNA viruses induces extensive remodeling of the host endomembrane system in favor of viral replication and movement. The integral membrane protein 6K2 of potyviruses induces the fo...
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Isolation and Single-Cell Transcriptomic Analysis of Murine Regulatory B Cells
Regulatory B (Breg) cells have been demonstrated to play an important role in the inhibition of a wide range of immunological responses, and they are absent or malfunction in autoimmune diseases like lupus. Br...
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Protocol
Method for the Enrichment of N6-Methyladenosine-Modified Cellular and HIV-1 RNA
N6-methyladenosine (m6A) modification of RNA is an important area in studying viral replication, cellular responses, and host immunity. HIV-1 RNA contains multiple m6A modifications that regulate viral replicatio...
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Protocol
Co-immunoprecipitation Assays to Detect Protein–Protein Interactions
Proteins usually do not function as monomers but rather perform their functions by interacting with themselves or other proteins. Co-immunoprecipitation is an essential assay for detecting protein interactions...
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Protocol
Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection
Measles virus (MeV) infection of airway surface epithelial cells provides a site for final amplification before being released back into the environment via coughing and sneezing. Multiple cell lines have serv...
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A Mouse Model of Non-Alcoholic Steatohepatitis and Hepatocellular Carcinoma Induced by Western Diet and Carbon Tetrachloride
Non-alcoholic steatohepatitis (NASH) is a severe form of non-alcoholic fatty liver disease (NAFLD). Obesity is a known risk factor of NASH, which, in turn, increases the risk of develo** cirrhosis (liver sca...
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Protocol
DeepHLApan: A Deep Learning Approach for the Prediction of Peptide-HLA Binding and Immunogenicity
Neoantigens are crucial in distinguishing cancer cells from normal ones and play a significant role in cancer immunotherapy. The field of bioinformatics prediction for tumor neoantigens has rapidly developed, ...
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Protocol
Biomarker Identification in Liver Cancers Using Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) Imaging: An Approach for Spatially Resolved Metabolomics
Liver cancers are characterized by interindividual and intratumoral heterogeneity, which makes early diagnosis and the development of therapies challenging. Desorption electrospray ionization mass spectrometry...
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Protocol
Evaluation of Oxygen Consumption Rates In Situ
An analysis of the mitochondrial respiration function represented by the oxygen consumption rate is necessary to assess mitochondrial bioenergetics and redox function. This protocol describes two alternative t...
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Protocol
A Robust In Vitro Co-culture Model for Studying the Intercellular Communication of Neutrophils
Communication among neutrophils plays critical roles during various phases of inflammatory responses, with clinical relevance to both acute and chronic inflammatory diseases. Despite its significance, underlyi...
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Protocol
Isolation and Culture of Mouse Primary Microglia and Oligodendrocyte Precursor Cells
Microglia and oligodendrocyte precursor cells (OPCs) are critical glia subsets in the central nervous system (CNS) and are actively engaged in a body of diseases, such as stroke, Alzheimer’s disease, multiple ...
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Protocol
CRISPR-Cas9-Induced Gene Editing in Primary Human Monocytes
Monocytes play important and diverse roles in both homeostatic and inflammatory immune responses. The CRISPR-Cas9 system in lentiviral vectors has been widely used to manipulate specific genes of immortal mono...
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Protocol
Proteomic Analyses of the Mouse Brain
Proteomics is a scientific field that aims to identify and characterize all proteins within a biological system, including their posttranslational modifications (PTMs), quantitative changes, and protein–protei...
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Protocol
TurboID-Based Proximity Labeling: A Method to Decipher Protein–Protein Interactions in Plants
Proteins form complex networks through interaction to drive biological processes. Thus, dissecting protein–protein interactions (PPIs) is essential for interpreting cellular processes. To overcome the drawback...
