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Protocol
Pilot Phase I Clinical Trial of Methioninase on High-Stage Cancer Patients: Rapid Depletion of Circulating Methionine
Methionine (MET) has been shown to be a tumor-selective therapeutic target for cancer, since cancer cells require higher amounts of MET to divide and survive than normal cells. This phenomena is known as MET d...
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Protocol
High Efficacy of Recombinant Methioninase on Patient-Derived Orthotopic Xenograft (PDOX) Mouse Models of Cancer
Methionine (MET) is a general target in cancer due to the excess requirement of MET by cancer cells. MET has been effectively restricted by recombinant methioninase (rMETase) in mouse models of cell-line tumor...
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Protocol
Development of Recombinant Methioninase for Cancer Treatment
The elevated requirement for methionine (MET) of cancer cells is termed MET dependence. To selectively target the MET dependence of tumors for treatment on a large-scale preclinical and clinical basis, the l-meth...
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Protocol
Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase
Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombina...
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Protocol
Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H2S Repair
Selenium is a biologically important trace element and acts as an active center of glutathione peroxidase (GPx). GPx is the important antioxidant enzyme to protect organisms from oxidative damage via catalyzin...
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Protocol
Multi-compartment Neuron–Glia Coculture Microsystem
Conventional cell culture plate based neuron–glia coculture methods have limitations in conducting localized physical and biochemical manipulations of cells that allow detailed investigation into axon–glia int...
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Protocol
Axon Length Quantification Microfluidic Culture Platform for Growth and Regeneration Study
To fully understand how external biomolecular environment influences axon growth, a method that can easily quantify the extent of axon growth as well as locally control their biomolecular environment is critic...
