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    Protocol

    Development of a Light-Dependent Protein Histidine Kinase

    Phosphorylation plays a critical role in facilitating signal transduction in prokaryotic and eukaryotic organisms. Our study introduces a tool for investigation of signal diffusion in a biochemical regulation ...

    Aleksandra E. Bury, Klaas J. Hellingwerf in Histidine Phosphorylation (2020)

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    Protocol

    On the In Vivo Redox State of Flavin-Containing Photosensory Receptor Proteins

    Measured values of the redox midpoint potential of flavin-containing photoreceptor proteins range from physiologically very negative values, i.e., < –300 mV (compared to the calomel electrode) for some LOV dom...

    Aleksandra Bury, Klaas J. Hellingwerf in Flavins and Flavoproteins (2014)

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    Protocol

    Prokaryotic Phototaxis

    Microorganisms have various mechanisms at their disposal to react to (changes in) their ambient light climate (i.e., intensity, color, direction, and degree of polarization). Of these, one of the best studied ...

    Wouter D. Hoff, Michael A. van der Horst, Clara B. Nudel… in Chemotaxis (2009)

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    Protocol

    8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein

    Fluorescence resonance energy transfer (FRET) is a technique that can be used to estimate intermolecular distances between pigment molecules, which is an approach first proposed by Stryer (1). The theoretical bas...

    Jasper van J. Thor, Klaas J. Hellingwerf in Green Fluorescent Protein (2002)