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Protocol
Deep Sequencing to Study Microbial Transcriptomic Responses to Hydrocarbon Degradation/Production/Stress
Until relatively recently, genome-wide bacterial transcription analysis has typically relied on oligonucleotide-based DNA microarrays. This approach, however, has drawbacks because of limitations of probe desi...
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Protocol
Detection of Specific mRNA Molecules in Hydrocarbon-Degrading Bacteria
A number of methods for visualization of specific mRNAs in bacterial cells have been developed recently, but not all of them are suitable for environmental species when examined under harsh growth conditions (...
