11 Result(s)
within
Protocol
Hongyan **g
-
Protocol
A DNA Pull-Down Assay with Diversity Forms of Competitor for Detecting or Evaluating Protein–DNA Interactions
DNA–protein interactions (DPIs) are critical to all living organisms, particularly in the regulation of gene expression, replication, packing, recombination, and DNA repair, as well as RNA transport and transl...
-
Protocol
Establishment of White-Backed Planthopper Cell Lines
The first continuous cell line of leafhopper was established over 50 years ago. Since then, leafhopper cell monolayers have been used extensively to assay the infectivity of plant viruses that multiply in thei...
-
Protocol
Characterizing Starch Molecular Structure of Rice
A better understanding of the nutritional properties of rice starch is important because of the rapid rise of diet-related health complications, particularly obesity, type 2 diabetes, and colorectal cancers. R...
-
Protocol
A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene
Given its increasing importance in transforming biomedical research in recent years, microarray technology has become highly popular as a powerful screening platform in detecting biomolecule interactions, disc...
-
Protocol
High-Throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) on Phosphopeptide Microarrays
Phosphatases are a family of enzymes responsible for the dephosphorylation of biomolecules. Phosphatases play essential roles in cell cycle regulation, signal transduction, and cellular communication. In recen...
-
Protocol
Online LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins
High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a gas-phase separation technique which, when coupled with liquid chromatography tandem mass spectrometry, offers benefits for analysis of com...
-
Protocol
Phosphopeptide Microarrays for Comparative Proteomic Profiling of Cellular Lysates
Protein phosphorylation is one of the most important and well-studied posttranslational modifications. Aberrant phosphorylation causes a wide spectrum of diseases, including cancers. As a result, many of the p...
-
Protocol
Chromosome-Wide Analysis of Protein Binding and Modifications
In order to fully understand the functions of a DNA-binding protein it is necessary to identify all of its binding sites in chromosomes and assess the role of each site in the overall biological function of th...
-
Protocol
Advanced In Vivo Heteronuclear MRS Approaches for Studying Brain Bioenergetics Driven by Mitochondria
The greatest merit of in vivo magnetic resonance spectroscopy (MRS) methodology used in biomedical research is its ability for noninvasively measuring a variety of metabolites inside a living organ. It, theref...
-
Protocol
CHK1 Kinase Activity Assay
Mammalian CHK1 is a Ser/Thr effector kinase that plays critical roles in the DNA damage-activated cell cycle checkpoint signaling pathway downstream of ATR (ATM and Rad3-related protein kinase). This chapter i...
-
Protocol
Direct In Situ Single-Copy (DISC)-PCR
The physical map** of functional genes and polymorphic markers is obviously important for understanding genome organization in higher organisms. Fluorescence in situ hybridization of labeled DNA to metaphase ch...
