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    Protocol

    Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae

    Changes in chromatin accessibility are an important aspect of the molecular changes that occur in eukaryotic cells responding to stress, and they appear to play a critical role in stress-induced transcriptiona...

    Yogendra Bordiya, Hong-Gu Kang in Plant Pattern Recognition Receptors (2017)

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    Protocol

    High-Throughput Targeted Transcriptional Profiling of Defense Genes Using RNA-Mediated Oligonucleotide Annealing, Selection, and Ligation with Next-Generation Sequencing in Arabidopsis

    Tracking RNA transcription has been one of the most powerful tools to gain insight into the biological process. While a wide range of molecular methods such as northern blotting, RNA-seq, and quantitative RT-P...

    Sung-Il Kim, Yogendra Bordiya, Ji-Chul Nam in Modeling Transcriptional Regulation (2021)

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    Protocol

    Co-immunoprecipitation for Assessing Protein–Protein Interactions in Agrobacterium-Mediated Transient Expression System in Nicotiana benthamiana

    The characterization of protein–protein interactions (PPI) often provides functional information about a target protein. Yeast-two-hybrid (Y2H) and luminescence/fluorescence-based detections, therefore, have b...

    Ji Chul Nam, Padam S. Bhatt, Sung-Il Kim, Hong-Gu Kang in Protein-Protein Interactions (2023)