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Protocol
Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells
Porcine epidemic diarrhea virus (PEDV), a single-stranded, positive-sense enveloped RNA virus, was first identified several decades ago and affects the pig industry worldwide. Virus isolation is essential for ...
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Protocol
CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing
The photoelectrochemical (PEC) biosensor, in which light is utilized to excite the photoactive species and current is employed as the detection signal, is a newly appeared yet dynamically develo** technique ...
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Protocol
Novel Random Mutagenesis Method for Directed Evolution
Directed evolution is a powerful strategy for gene mutagenesis, and has been used for protein engineering both in scientific research and in the biotechnology industry. The routine method for directed evolutio...
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Protocol
Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) represent a main population of stem cells and can differentiate into multiple cell lineages. Recently, MSC transplantation has been applied to repair the malfunctioned tissues. Ho...