6 Result(s)
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Protocol
Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Microdissection
Tumors are heterogeneous structures that contain different cell populations. Laser capture microdissection (LCM) can be used to obtain pure cancer cells from fresh-frozen cancer tissue and the surrounded envir...
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Protocol
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris, using a secretory vector. A truncated ...
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Protocol
Detection of Differentially Expressed Genes in Cancer Using Differential Display
It is estimated that the human genome contains 25,000–35,000 genes; however, only about 15% of these are expressed in any given cell and different cells and tissues express different gene subsets. Gene express...
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Protocol
Methods for Chemoprotection and Chemosensitization
Surgery, radiation therapy, and chemotherapy have been applied to the curative therapy of 50% of cancer patients in the United States during the past 100 years. It is clear that the chemotherapeutic agents use...
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Protocol
Anti-MDR1 Ribozyme Gene Therapy
Multidrug resistance (MDR) in human cancer seriously limits the efficacy of anticancer agents. Circumvention of MDR is, thus, one of the urgent goals for successful cancer chemotherapy.
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Protocol
Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
Electrophoresis is an important and fundamental tool for DNA analysis. Traditional DNA electrophoresis is labor-intensive, skill-dependent, and relatively slow. To achieve greater performance, capillary electr...
