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    Protocol

    Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode

    As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid ...

    Alyah Buzid, John H. T. Luong, F. Jerry Reen, Fergal O’Gara in Quorum Sensing (2018)

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    Protocol

    Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites

    The advent of metagenomics based biodiscovery has provided researchers with previously unforeseen access to the rich tapestry of natural bioactivity that exists in the biosphere. Unhindered by the “culturable ...

    F. Jerry Reen, Jose A. Gutiérrez-Barranquero, Fergal O’Gara in Metagenomics (2017)

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    Protocol

    Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay

    The common cardiovascular, respiratory, gastrointestinal tract, renal, and immune diseases share common etiologies, because they are multifactorial in origin, with both genetic and environmental components con...

    Frank Waldron-Lynch, Claire Adams, Michael G. Molloy, Fergal O’Gara in RT-PCR Protocols (2002)