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Protocol
Three-Dimensional Assessment of Crown Size and Eruption Space for Develo** Third Molars: Data Collection Techniques Based on Cone-Beam Computed Tomography (CBCT)
Third molar development and eruption are two related areas of major interest in dental research into the etiology of “wisdom tooth” impaction. Third molars are not only an excellent model for studying dental d...
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Protocol
Targeting HER2 in Advanced Breast Cancer
Human epidermal growth factor receptor 2 (HER2) is a human oncogene that is amplified in approximately 20% of breast cancers, and portends a worse prognosis if not treated with anti-HER2 agents. The advent of tar...
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Protocol
Primary Choroid Plexus Tissue for Use in Cellular Therapy
The choroid plexus (CP) has been explored as a cellular therapeutic due to its broad-ranging secretome and demonstrated longevity in a variety of encapsulation modalities. While the CP organ is normally involv...
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Protocol
LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks
Protein–protein interactions (PPIs) play an essential role in all biological processes. In vivo, PPIs occur dynamically and depend on extracellular cues. To discover novel protein–protein interactions in mamma...
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Protocol
T2 Magnetic Resonance for Fungal Diagnosis
Rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. T2Candida panel is a novel qualitative diagnostic p...
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Protocol
Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism
Cell encapsulation is an alternative to avoid rejection of grafted tissue, thus bringing an interesting alternative in cell therapy. It is particularly relevant in ailments where only the implant of small quan...
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Protocol
Method for Analysis of Matrix Degradation by CCN2 Through the MMP/TIMP System
Many studies have shown effects of members of the CCN family on matrix synthesis and accumulation but few have examined degradative pathways. This scarcity of information is in part due to the lack of suitable...
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Protocol
Therapeutic Application of Adult Stem Cells in the Heart
Cell therapies have been explored as a potential treatment avenue to treat heart diseases, such as myocardial infarction, doxorubicin-induced cardiomyopathy, and heart failure. Embryonic and adult stem cells (...
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Protocol
Methods for Molecular Diagnosis of Human Prion Disease
Human prion diseases are associated with a range of clinical presentations, and they are classified by both clinicopathological syndrome and etiology, with subclassification according to molecular criteria. He...
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Protocol
In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma
The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has i...
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Protocol
Measurement of Angiotensin Peptides: HPLC-RIA
The renin-angiotensin system (RAS) is a complex circulating and tissue-based system. There are multiple pathways for the formation and degradation of peptides. In order to understand the functions of the syste...
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Protocol
Innovations in Clinical Trial Design in the Era of Molecular Profiling
Historically, cancer has been studied, and therapeutic agents have been evaluated based on organ site, clinical staging, and histology. The science of molecular profiling has expanded our knowledge of cancer a...
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Protocol
Critical Periods of Development in Teratology
Several years ago Wilson (1973) postulated his principles of teratogenesis, which have been revisited previously. However, there is a strong need to evaluate critically and summarize recent advances in the are...
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Protocol
Peptide-Based Cancer Vaccine Strategies and Clinical Results
Active cancer immunotherapy is an exciting and develo** field in oncology research. Peptide vaccines, the use of isolated immunogenic tumor-associated antigen (TAA) epitopes to generate an anticancer immune ...
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Protocol
Protocols for in vitro Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and Adipogenic Lineages
Mesenchymal stem cells (MSC) possess high plasticity and the potential to differentiate into several different cell types; this characteristic has implications for cell therapy and reparative biotechnologies. ...
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Protocol
Urine Purine Metabolite Determination by UPLC-Tandem Mass Spectrometry
Inborn errors of purine metabolism, either deficiencies of synthesis or catabolism pathways, lead to a wide spectrum of clinical presentations: urolithiasis (adenine phosphoribosyltransferase), primary immune ...
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Protocol
Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
Succinylacetone (SA) is used for the diagnosis and monitoring of patients with tyrosinemia type I (Tyr I). SA is exclusively elevated in blood and urine of patients with Tyr I. As urinary SA concentration is m...
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Protocol
How to Avoid Bum** into the Translational Roadblock
Translating neuroprotective efficacy from animal studies to clinical trials in humans has been fraught with difficulty. This failure might be because animal studies were falsely positive or because clinical tr...
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Protocol
Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
Pyrimidine diseases result from deficiencies in pyrimidine de novo synthesis, degradation, and salvage pathways. Enzymatic deficiencies in pyrimidine catabolism lead to mitochondrial neurogastrointestinal ence...
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Protocol
Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium
Mesenchymal stem cells (MSC) produce and secrete a great variety of cytokines and chemokines that play beneficial paracrine actions when MSC are used for tissue repair. The conditioned medium (CM) derived from...
