23 Result(s)
within
Protocol
Baohong **
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Protocol
Impact of Nanoparticles on Plant Growth, Development, and Biomass
Nanoparticles are an extensive class of naturally occurred or man-made objects; it has been widely using in our daily life. As increasing usage, nanoparticles are also released into the environment and are bec...
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Protocol
QuickIsoSeq for Isoform Quantification in Large-Scale RNA Sequencing
RNA-sequencing (RNA-seq) is a powerful technology for transcriptome profiling. While most RNA-seq projects focus on gene-level quantification and analysis, there is growing evidence that most mammalian genes a...
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Protocol
Biochemical and Physiological Toxicity of Nanoparticles in Plant
As increasing application of nanoparticles, nanoparticles have been becoming a new emerging environmental pollution that attracts a lot of attention from the scientific community and also regulatory agents. In...
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Protocol
Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis
Gene expression analysis has been becoming a popular method for studying gene function and response to different environmental stresses, including toxin/pollution exposure. Selection of a suitable reference ge...
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Protocol
A Grafting Technique for Efficiently Transplanting Transgenic Regenerated Plants of Cotton
Recovery of transgenic cotton plants from tissue culture condition to greenhouse condition is a critical step for improving cotton through genetic engineering. Traditional methods always cause low survival rat...
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Protocol
Pollen Tube Pathway-Mediated Cotton Transformation
Although many gene transfer methods have been employed for successfully obtaining transgenic cotton, the major constraint in cotton improvement is the limitation of genotype because the majority of transgenic ...
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Protocol
Genome Editing in Cotton Using CRISPR/Cas9 System
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is emerging as effective strategy for generating site-specific mutations. Recently, CRISPR/Cas9-me...
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Protocol
A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene
Determining transgenic events is a critical step for obtaining transgenic plants as well as the later stage of application. Traditional methods, such as Northern blotting and qRT-PCR, for determining transgeni...
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Protocol
Overexpression of miRNA in Cotton via Agrobacterium-Mediated Transformation
microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progress through t...
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Protocol
Agrobacterium-Mediated Genetic Transformation of Cotton
There are many methods and techniques that can be used to transfer foreign genes into cells. In plant biotechnology, Agrobacterium-mediated transformation is a widely used traditional method for inserting foreign...
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Protocol
Tobacco Rattle Virus-Induced Gene Silencing in Cotton
Virus-induced gene silencing (VIGS), as a tool for plant reverse genetics, has been widely used in cotton for target gene function analysis. Compared with genetically transformed plants, the target gene expres...
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Protocol
Transgenic Cotton: From Biotransformation Methods to Agricultural Application
Transgenic cotton is among the first transgenic plants commercially adopted around the world. Since it was first introduced into the field in the middle of the 1990s, transgenic cotton has been quickly adopted...
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Protocol
QuickRNASeq: Guide for Pipeline Implementation and for Interactive Results Visualization
Sequencing of transcribed RNA molecules (RNA-Seq) has been used wildly for studying cell transcriptomes in bulk or at the single-cell level (Wang et al., Nat Rev Genet, 10:57–63, 2009; Ozsolak and Milos, Nat R...
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Protocol
Using Quantitative Real-Time PCR to Detect MicroRNA Expression Profile During Embryonic Stem Cell Differentiation
Quantitative real-time PCR (qRT-PCR) is a reliable method to determine and monitor microRNA (miRNA) expression profiles in different cells, tissues, and organisms. Although there are several different strategi...
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Protocol
An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants
Recovery of transgenic cotton plants from tissue culture condition to greenhouse condition is a critical step for improving cotton through genetic engineering. Traditional methods always cause low survival rat...
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Protocol
Cotton Transformation via Pollen Tube Pathway
Although many gene transfer methods have been employed for successfully obtaining transgenic cotton, the major constraint in cotton improvement is the limitation of genotype because the majority of transgenic ...
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Protocol
A Simple and Rapid Method for Determining Transgenic Cotton Plants
Determining transgenic events is a critical step for obtaining transgenic plants as well as the later stage of application. Traditional methods, such as Northern blotting and qRT-PCR, for determining transgeni...
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Protocol
Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation
microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progresses through...
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Protocol
Transgenic Cotton: From Biotransformation Methods to Agricultural Application
Transgenic cotton is among the first transgenic plants commercially adopted around the world. Since it was first introduced into the field in the middle of 1990s, transgenic cotton has been quickly adopted by ...
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Protocol
Agrobacterium-Mediated Transformation of Cotton
There are many methods and techniques that can be used to transfer foreign genes into cells. In plant biotechnology, Agrobacterium-mediated transformation is a widely used traditional method for inserting foreign...
