2,174 Result(s)
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Protocol
Protocol for Testing the Effects of ssDNA Aptamer in HeLa and MCF-7
Conventional approaches for treating tumors encompass chemotherapy, radiotherapy, and surgery. However, these methods come with their limitations when applied in clinical practice. Aptamers are often referred ...
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Protocol
A Practical Approach to Using the Genomic Standards Consortium MIxS Reporting Standard for Comparative Genomics and Metagenomics
Comparative analysis of (meta)genomes necessitates aggregation, integration, and synthesis of well-annotated data using standards. The Genomic Standards Consortium (GSC) collaborates with the research communit...
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Protocol
Replication Assay of Begomovirus in Arabidopsis Protoplasts
Protoplasts are isolated plant cells from which the cell walls have been removed by treatment with fungal cellulase and macerozyme enzymes, which degrade the primary components of the cell wall. The protoplast...
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Protocol
Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins
Many negative-sense single-stranded RNA viruses within the order Mononegavirales harm humans. A common feature shared among cells infected by these viruses is the formation of subcellular membraneless structures ...
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Protocol
RNA Isolation from Ctenophores
RNA-seq or transcriptome analysis of individual cells and small cell populations is essential for virtually any biomedical field. Here, we examine and discuss the different methods of RNA isolation specific to...
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Protocol
Frequencies of SARS-CoV-2 Spike Protein-Specific Memory B Cells in Human PBMCs, Quantified by ELISPOT Assay
Vaccination against SARS-CoV-2 with coronavirus vaccines that elicit protective immune responses is critical to the prevention of severe disease and mortality associated with SARS-CoV-2 infection. Understandin...
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Protocol
DNA Methylation in Ctenophores
Epigenomic regulation and dynamic DNA methylation, in particular, are widespread mechanisms orchestrating the genome operation across time and species. Whole-genome bisulfite sequencing (WGBS) is currently the...
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Protocol
Species Identification Through Sequencing
Ciliates with nuclear dualism are reported to be a highly divergent unicellular eukaryotes. They play very vital roles in regulation of microbial food webs as they feed on bacteria, protists, and even on micro...
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Protocol
Comprehensive Urine Drug Screen by Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry
Current trends in drug use include polydrug use as well as an ever-changing landscape of novel psychoactive substances. Immunoassay-based urine drug screens limited to classic drugs of abuse do not have the sc...
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Protocol
Manipulation of Proteostasis Networks in Transgenic ZAAT Zebrafish via CRISPR–Cas9 Gene Editing
The CRISPR–Cas9 genome editing system is used to induce mutations in genes of interest resulting in the loss of functional protein. A transgenic zebrafish α1-antitrypsin deficiency (AATD) model displays an unu...
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Protocol
Determining Isoprenoid-Facilitated Monomeric GTPase Turnover in Primary Human Trabecular Meshwork Cultures
Members of the Rho family of small monomeric GTPases regulate a plethora of critical cellular functions including gene expression, cell cycle progression, and the dynamic modeling of the actin cytoskeleton. Di...
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Protocol
Purification of Phytaspases Using a Biotinylated Peptide Inhibitor
Phytaspases are plant subtilisin-like proteases (subtilases) that possess a rarely occurring substrate cleavage specificity. These proteolytic enzymes hydrolyze their substrates strictly after an aspartate res...
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Protocol
A Method to Conditionally Measure Target Engagement at Intracellular RAS and RAF Complexes
Dysfunction of the RAS/mitogen-activated protein kinase (MAPK) pathway is a common driver of human cancers. As such, both the master regulator of the pathway, RAS, and its proximal kinase effectors, RAFs, have...
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Protocol
Use of Surface Plasmon Resonance Technique for Studies of Inter-domain Interactions in Ion Channels
Ion channels are transmembrane proteins essential for cellular functions and are important drug targets. Surface plasmon resonance (SPR) is a powerful technique for investigating protein–protein and protein–sm...
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Protocol
Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers
Micro- and nanoparticles are often designed by mimicking naturally occurring structures. Bacterial spores are dormant cells elaborated by some Gram-positive bacteria during poor growth conditions to protect th...
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Protocol
Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans After Developmental Lead Exposure
The roundworm Caenorhabditis elegans (C. elegans) has become a powerful tool to evaluate the deleterious effects of early-life exposure to xenobiotics, including metals. The present chapter describes a detailed p...
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Protocol
A Semiautomated Proteomics and Phosphoproteomics Protocol for the Identification of Novel Therapeutic Targets and Predictive Biomarkers in In Vivo Xenograft Models of Pediatric Cancers
Genomic profiling has identified therapeutic targets for precision treatment of certain cancers, but many patients lack actionable mutations. Additional omics approaches, like proteomics and phosphoproteomics,...
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Protocol
Untargeted High-Resolution Mass Spectrometry for Detection of Natural Products and Confiscated Powders and Pills
Drug testing for clinical toxicology purposes is difficult given the variety of drugs and substances that can be abused. High-resolution mass spectrometry is a powerful tool for the qualitative identification ...
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Protocol
Quantitative FISHing: Implications for Chromosomal Analysis
Quantifying signals substantially increases the efficiency of fluorescence in situ hybridization (FISH). Quantitative FISH analysis or QFISHing may be useful for differentiation between chromosome loss and chr...
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Protocol
Quantitative Assay of Fentanyl and Analogs Using LC-MS/MS
Drug overdoses and drug-involved deaths continue to increase in the United States, with most drug overdose deaths involving . Among drug overdose deaths, , particularly fentanyl and its analogs, remain the m...
