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Protocol
Map** Meiotic DNA Breaks: Two Fully-Automated Pipelines to Analyze Single-Strand DNA Sequencing Data, hotSSDS and hotSSDS-extra
Molecular approaches are required to detect DNA double-strand break (DSB) events and to map and quantify them at high resolution. One of the most popular molecular methods in the field of meiotic recombination...
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Protocol
Atypical Development of Tactile Processing
Tactile processing is of tremendous importance during development. Touch is one of the first senses to develop, and is even active prenatally. Touch allows children not only to explore the physical world, but ...
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Protocol
A Practical Guideline to Engineering Nonribosomal Peptide Synthetases
The bioengineering of nonribosomal peptide synthetases (NRPSs) is a rapidly develo** field to access natural product derivatives and new-to-nature natural products like scaffolds with changed or improved pro...
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Protocol
Associating Aversive Task Exposure with Pharmacological Intervention to Model Traumatic Memories in Laboratory Rodents
Post-traumatic stress disorder is associated with highly threatening and stressful events. The underlying memory is overconsolidated, leading to generalized fear expression and overall resistance to extinction...
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Protocol
The Effect of Oxytocin on Intracellular Ca2+ Release in Cardiac Cells
Ca2+ signaling is vital for the proper functioning of all cells, including cells of the cardiovascular system. Membrane receptors for many hormones trigger intracellular Ca2+ signaling via the activation of phosp...
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Protocol
Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections
Pathogens such as Staphylococcus aureus are able to survive in many types of host cells including phagocytes such as neutrophils and macrophages, thereby resulting in intracellular infections. Treatment of intrac...
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Protocol
Functional Hemolytic Test for Complement Alternative Pathway Convertase Activity
The complement system is a key part of innate immunity. However, if the system becomes dysregulated, damage to healthy host cells can occur, especially to the glomerular cells of the kidney. The convertases of...
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Protocol
Biotinylation of Membrane Proteins for Binder Selections
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the ...
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Protocol
A Biomimetic Model for Mineralization of Type-I Collagen Fibrils
The bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In vitro biomimetic models based on self-assembled collagen fibrils have been widely used in stud...
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Protocol
Antimicrobial Susceptibility Testing Methods for Acinetobacter spp.
Serial twofold dilution methods are most commonly used to identify the antimicrobial activities of antibiotics. This can be achieved by different methods like broth dilution or agar dilution. Though these meth...
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Protocol
Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimal CSF Protein Biomarkers
The field of neurological diseases strongly needs biomarkers for early diagnosis and optimal stratification of patients in clinical trials or to monitor disease progression. Cerebrospinal fluid (CSF) is one of...
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Protocol
Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo
Over the last decades, it has been established that the immune system is crucial for the impediment of cancer development by recognizing and destroying transformed cells. This process has been termed cancer im...
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Protocol
The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer
Advances in embryo technologies in the domestic dog have made significant strides in the past decade. This progress has been spurred by interests in taking advantage of the dog as a biomedical research model f...
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Protocol
Erratum to: Large-Brained Animal Models of Huntington’s Disease: Sheep
The name of the Chapter 12 author had been inadvertently mentioned as “Jennifer Morton A”. This has been correctly updated as “Morton. A. J”.
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Protocol
Quantum Cascade Lasers-Based Detection of Nitric Oxide
Despite the established importance of nitric oxide (NO) in many physiological and molecular processes in plants, most methods for quantifying NO are open to criticism This reflects the differing methods either...
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Protocol
Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors
Activation of the heat shock response, and in particular upregulation of stress-inducible Hsp70, herein referred to as Hsp70i, in newly transformed cells, appears to protect against protein damaging stimuli, i...
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Protocol
Large-Brained Animal Models of Huntington’s Disease: Sheep
The limitations of using small-brained rodents to model diseases that affect large-brain humans are becoming increasingly obvious as novel therapies emerge. Huntington’s disease (HD) is one such disease. In re...
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Protocol
Anaerobic Digestion of Lipid-Rich Waste
Lipids present in waste and wastewater, also referred as fat, oil, and grease (FOG), can be efficiently converted to methane. This fact constitutes an opportunity for conserving the high energy content of wast...
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Protocol
Measuring Mitochondrial Pyruvate Oxidation
Pyruvate is a central metabolic intermediate and plays a prominent role in nervous system function. Neurons are highly reliant on pyruvate oxidation for maintenance of cellular energetics. Disorders in pyruvat...
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Protocol
Laser-Based Methods for Detection of Nitric Oxide in Plants
Nitric oxide (NO) plays an important role in plant signaling and in response to various stress conditions. Therefore, real-time measurements of NO production provide better insights into understanding plant pr...
