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Protocol
Predicting Pseudouridine Sites with Porpoise
is a ubiquitous modification and plays a crucial role in many biological processes. However, it remains a challenging task to identify sites using expensive and time-consuming experimental research. To th...
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Protocol
Quantitative Analysis of Directional Neural Crest Cell Migration
The neural crest is a highly migratory cell population that evolved in vertebrates. Born at the lateral borders of the neural plate, neural crest cells migrate long distances along defined paths and contribute...
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Protocol
Systematic Characterization of Lysine Post-translational Modification Sites Using MUscADEL
Among various types of protein post-translational modifications (PTMs), lysine PTMs play an important role in regulating a wide range of functions and biological processes. Due to the generation and accumulat...
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Protocol
Experimental and Computational Workflow for RNA Sequencing in Mycobacterium tuberculosis : From Total RNA to Differentially Expressed Genes
RNA sequencing (RNAseq) in bacteria has become a transformative tool for many applications, including the identification of mechanisms that contribute to pathogenesis, environmental adaptation, and drug respon...
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Protocol
Fabrication and Electrospinning of 3D Biodegradable Poly-l-Lactic Acid (PLLA) Nanofibers for Clinical Application
Poly-l-lactic acid (PLLA) is a biodegradable synthetic polyester synthesized by polymerization or polycondensation. PLLA hydrolytically degrades into lactic acid, a biocompatible metabolic by-product, making it s...
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Protocol
Molecular Identification of Transmitted/Founder Hepatitis C Viruses and Their Progeny by Single Genome Sequencing
Chronic hepatitis C virus (HCV) infection exists as a complex mixture of genetically distinct viruses, commonly referred to as a “quasispecies.” Quasispecies complexity can vary substantially during the course...
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Protocol
Analysis of Mitochondrial Markers of Programmed Cell Death
Mitochondria play a crucial role in programmed cell death (PCD) in plants. In most cases of mitochondria-dependent PCD, cytochrome c (Cyt c) released from mitochondria due to the opening of mitochondrial perme...
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Protocol
Rapid Detection of Brucella spp. Using Loop-Mediated Isothermal Amplification (LAMP)
Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Livestock that are most vulnerable to brucellosis include cattle, goats, and pigs. Bruce...
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Protocol
Visual Evoked Potential Recording in Rodents
The visual evoked potential (VEP) is an electronic potential recorded from the visual cortex in response to a visual stimulus. It provides a means to examine the function of the visual pathway from the retina ...