10 Result(s)
within
Protocol
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Protocol
sRNA Structural Modeling Based on NMR Data
Small non-coding RNAs (sRNAs) play vital roles in gene expression regulation and RNA interference. To comprehend their molecular mechanisms and develop therapeutic approaches, determining the accurate three-di...
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Fluorine (19F) MRI to Measure Renal Oxygen Tension and Blood Volume: Experimental Protocol
Fluorinated compounds feature favorable toxicity profile and can be used as a contrast agent for magnetic resonance imaging and spectroscopy. Fluorine nucleus from fluorinated compounds exhibit well-known adva...
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Application of NMR Spectroscopy to Determine Small RNA Structure
Nuclear magnetic resonance (NMR) spectroscopy is a key experimental method to investigate the structure and dynamics of RNA. RNA often has only partially ordered structures responsible for its function, which ...
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Computational Prediction of Protein O-GlcNAc Modification
Protein O-GlcNAcylation on serine and threonine residues is a significant posttranslational modification. Experimental techniques can uncover only a small portion of O-GlcNAcylation sites. Several computationa...
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Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD...
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In Utero Delivery of Oligodeoxynucleotides for Gene Correction
Gene correction is attractive for single gene mutation disorders, such as Duchenne muscular dystrophy (DMD). The mdx mouse model of DMD is dystrophin deficient due to a premature chain-terminating point mutation ...
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Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus
Monoclonal antibodies to specific vaccinia virus (VACV) proteins are valuable reagents in studies of VACV. In this chapter, we describe methods of generating a panel of monoclonal antibodies that recognize a v...
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General Consideration on Sialic Acid Chemistry
Sialic acids, also known as neuraminic acids, are a family of negatively charged α-keto acids with a nine-carbon backbone. These unique sugars have been found at the termini of many glycan chains of vertebrate...
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Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes
Replication of the genome of positive-strand RNA plant viruses takes place in membrane-bound complexes that contain viral replicase proteins, viral RNA, and host proteins. Many viral replicase proteins play a ...
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Establishment of a Cell-Free System to Study the Activation of Chk2
The checkpoint kinase Chk2 is activated in response to DNA damage through pathways requiring protein kinases ATM and/or ATR. The means by which Chk2 is activated by these kinases still remains to be addressed....
