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    Protocol

    Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD+/NADH-Specific Fluorescent Protein Sensors

    The pyridine nucleotides NAD(H) and NADP(H) are key molecules in cellular metabolism, and measuring their levels and oxidation states with spatiotemporal precision is of great value in biomedical research. Tra...

    Cláudio F. Costa, Hongli Li, Mohamed A. F. Hussein, Yi Yang, Celien Lismont… in Peroxisomes (2023)

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    Protocol

    Identification of Peroxisome-Derived Hydrogen Peroxide-Sensitive Target Proteins Using a YAP1C-Based Genetic Probe

    As the reversible oxidation of protein cysteine thiols is an important mechanism in signal transduction, it is essential to have access to experimental approaches that allow for spatiotemporal indexing of the ...

    Celien Lismont, Iulia Revenco, Cláudio F. Costa, Hongli Li… in Peroxisomes (2023)