6 Result(s)
within
Protocol
**ngguo **ao
Life Sciences
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Protocol
Generation of Organoids and Analysis of Ferroptosis in Organoids
Ferroptosis is a unique form of iron-dependent cell death induced by lipid peroxidation and subsequent plasma membrane rupture, which sets it apart from other types of regulated cell death. Ferroptosis has bee...
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Protocol
Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells
Mesenchymal stem/stromal cells (MSCs) show excellent therapeutic potentials in many preclinical studies and clinical trials. However, the clinical application of conventional tissue-derived MSCs faces challeng...
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Protocol
Cloning and Characterization of Argonaute Genes in Tomato
Argonaute (AGO) proteins are core elements in plant posttranscriptional RNA silencing pathways. The identification and functional characterization of tomato (Solanum lycopersicum) AGOs will help to better underst...
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Protocol
Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
Small noncoding RNAs are essential for gene expression at transcriptional and posttranscriptional levels. Northern blot is the most used method for small RNA detection in tissues. Here we present an improved p...
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Protocol
Mannose-6-Phosphate: A Regulator of LLO Destruction
Oligosaccharyltransferase (OT) catalyzes the signature reaction of the asparagine-linked glycosylation pathway, namely, the transfer of preformed glycans from the lipid-linked oligosaccharide Glc3Man9GlcNAc2-P...
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Protocol
Application of Fluorophore-Assisted Carbohydrate Electrophoresis for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues
Defects in the synthesis of dolichol-linked oligosaccharide (or lipid-linked oligosaccharide [LLO]) cause severe, multisystem human diseases called type 1 congenital disorders of glycosylation (CDG type 1). LL...
