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Protocol
High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy
Brain is composed of complex networks of neurons that work in concert to underlie the animal’s cognition and behavior. Neurons communicate via structures called synapses, which typically require submicron spat...
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Protocol
AIRR Community Guide to Planning and Performing AIRR-Seq Experiments
The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last dec...
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Protocol
Bulk gDNA Sequencing of Antibody Heavy-Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community
In this method we illustrate how to amplify, sequence, and analyze antibody/immunoglobulin (IG) heavy-chain gene rearrangements from genomic DNA that is derived from bulk populations of cells by next-generatio...
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Protocol
MR Elastography of the Abdomen: Basic Concepts
Magnetic resonance elastography (MRE) is an emerging imaging modality that maps the elastic properties of tissue such as the shear modulus. It allows for noninvasive assessment of stiffness, which is a surroga...
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Protocol
MR Elastography of the Abdomen: Experimental Protocols
Application of MRE for noninvasive evaluation of renal fibrosis has great potential for noninvasive assessment in patients with chronic kidney disease (CKD). CKD leads to severe complications, which require di...
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Protocol
Erratum to: Multiresidue Pesticide Analysis by Capillary Gas Chromatography-Mass Spectrometry