1,463 Result(s)
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Protocol
A Coculture System for Modeling Intestinal Epithelial-Fibroblast Crosstalk
Epithelial organoid monoculture is a powerful tool to model stem cell dynamics in vitro. However, extensive efforts have recently revealed various niche players and their significant roles in regulating epithe...
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Protocol
Isolation of Mitochondria from Murine Skeletal Muscle
Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body’s metabolic homeostasis, skeletal muscle is also a target of many pathological conditions....
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Protocol
Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method
Adipose tissue provides a valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely used adipose-derived stromal/stem cells (ASCs) isolation protoc...
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Protocol
Integrating HexNAcQuest with Glycoproteomics Data Analysis Software to Distinguish HexNAc Isomers on Peptides
Recently, HexNAcQuest was developed to help distinguish peptides modified by HexNAc isomers, more specifically O-linked β-N-acetylglucosamine (O-GlcNAc) and O-linked α-N-acetylgalactosamine (O-GalNAc, Tn antig...
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Protocol
Isolation of Human Adipose-Derived Stromal/Stem Cells from Lipoaspirates
Adipose tissue is an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Adipose-derived stromal/stem cells (ASCs) ha...
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Protocol
Quantitation of Bupropion in Urine by Liquid Chromatography-Tandem Mass Spectrometry
Bupropion is a medication commonly used to treat depression and help people quit smoking. However, it has the potential to be misused or diverted, so it is important to have accurate and sensitive methods to d...
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Protocol
Single-Nucleus ATAC-seq for Map** Chromatin Accessibility in Individual Cells of Murine Hearts
During the last decade a wide range of single-cell and single-nucleus next-generation sequencing techniques have been developed, which revolutionized detection of rare cell populations, enabling creation of co...
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Protocol
Investigating the Effects of Temperature on Pathogen Propagation in Arabidopsis
Temperature is one of the most prominent environmental factors that influence plant immunity. Depending on the plant-pathogen system, increased temperature may inhibit or enhance disease resistance or immunity...
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Protocol
Orthotopic Model of Hepatocellular Carcinoma in Mice
Orthotopic models of hepatocellular carcinoma (HCC) consist in the implantation of tumor cells into the liver by direct intrahepatic injection. In this model, tumorigenesis is triggered within the hepatic micr...
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Protocol
Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy
Tracking microscopy enables whole-brain cellular resolution imaging in freely swimming animals. This technique enables both structural and functional imaging without immobilizing the animal, and greatly expand...
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Protocol
Two-Photon FRET/FLIM Imaging of Cerebral Neurons
Two-photon FRET (Förster resonance energy transfer) and FLIM (fluorescence lifetime imaging microscopy) enable the detection of FRET changes of fluorescence reporters in deep brain tissues, which provide a val...
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Protocol
Human Pre-gastrulation Embryo Culture in 3D Condition
The development process of human embryo until blastocyst is well understood during the past 30 years, however, embryogenesis from blastocyst to pre-gastrulation was still remained a “black box”. Limited by res...
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Protocol
The Direct Symbiotic Germination of Orchid Seeds in the Field
Mycorrhizal fungi are essential to the sustainability of the orchid population. Due to the minute nature of seeds, the mycobiont that the seedling can parasitize is considered an important element. Mycobionts ...
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Protocol
Analysis of Raffinose Metabolism-Related Sugar Components in Seeds
Raffinose family oligosaccharides (RFOs) are synthesized from sucrose and subsequent addition of galactose moieties which was provided by galactinol. Galactinol is synthesized from UDP-galactose and myo-inositol....
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Protocol
A Mouse Model of Hepatocellular Carcinoma Induced by Streptozotocin and High-Fat Diet
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and the second most common cause of cancer-related death. HCC is associated to chronic diseases such as viral hepatitis, alcoholic, and no...
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Protocol
Single-Cell Imaging of mRNA by Target RNA-Initiated RCA
We present a powerful method for direct mRNA detection based on ligation-based recognition and in situ amplification, capable of single-cell imaging mRNA at single-nucleotide and single-molecule resolution. At...
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Protocol
Profiling Human Cerebrospinal Fluid (CSF) Endogenous Peptidome in Alzheimer’s Disease
Human cerebrospinal fluid (CSF) is a rich source for central nervous (CNS)-related disease biomarker discovery due to its direct interchange with the extracellular fluid of the CNS. Though extensive proteome...
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Protocol
Targeted Analysis of Glycerophospholipids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer
The metabolic rearrangements of hepatic metabolism associated with liver cancer are still incompletely understood. There is an ongoing need to identify novel and more efficient diagnostic biomarkers and therap...
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Protocol
Automated Real-Time Monitoring of Extracellular pH to Assess Early Plant Defense Signaling
Extracellular alkalinization mediated by the inhibition of plasma membrane-located proton pum** ATPases hallmarks the initiation of defense signaling in plant cells. Early defense responses also include depo...
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Protocol
Quantitation of Urine Nicotine, Cotinine, and 3-OH-Cotinine by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Nicotine is a naturally occurring and highly addictive chemical used in e-cigarettes, cigarettes, chewing tobacco, and other tobacco products as well as in nicotine replacement therapies. The negative health c...
