2,871 Result(s)
within
Protocol
**e **an
Molecular Medicine
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Protocol
Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators
Omega-3 polyunsaturated fatty acids (PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) display beneficial actions in human diseases. The molecular basis for these actions remains of int...
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Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy
Adhesive interactions between bacterial cells coupled with adherence to a solid surface can lead to the formation of a biofilm. The important role of biofilm formation in the pathogenesis of certain types of i...
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Simultaneous Immunofluorescence Detection of Cadherin and β-Catenin in Mouse Tissue Sections
Both Cell-Cell adhesion and secreted signaling molecules are involved in the regulation of normal tissue development and maintenance. β-catenin seems to link cadherin-mediated Cell-Cell adhesion and Wnt signal...
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Nucleotide Metabolism and Cell-Cell Interactions
Interactions between the vascular endothelium and polymorphonuclear leukocytes (PMNs) are central to PMN emigration into inflamed tissues, and to neutrophil–endothelial crosstalk pathways that modulate inflammato...
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A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum
Asiatic cholera is a rapidly progressing disease resulting in extreme diarrhea and even death. The causative agent, cholera toxin, is an AB5 -subunit enterotoxin produced by the bacterium Vibrio cholera. The toxi...
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Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
The inflammatory response is a critical component of host defense. An important goal of our group has been to understand the endothelial-dependent mechanisms that mediate leukocyte recruitment during an inflam...
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Isolation and Analysis of Lipid Rafts in Cell–Cell Interactions
Lipid rafts are dynamic structures made up of proteins and lipids that float freely within the liquid-disordered bilayer of cellular membranes and have the ability to cluster to form larger, more-ordered platf...
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Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization
The interactions between egg and sperm are among the most fascinating in cell biology. These interactions include Cell-Cell adhesion and then membrane fusion between the two gametes. This chapter details the e...
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Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences
Epithelial cells at all mucosal surfaces are potentially apposed to bacteria, particularly in the intestine. It is established that intestinal epithelial cells (IECs) represent an important barrier between lam...
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Expression Cloning of Signaling Proteins Regulated by Cell Adhesion
Many proteins involved in Cell-Cell and cell–matrix adhesion are regulated by signal transduction pathways and can activate signal transduction on ligation. Adhesion-related signal transduction is important th...
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Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements
This chapter describes in vitro methods for studying the Blood-Brain barrier. These methods include a cell line and isolated brain microvessels. The rat brain endothelial cell line 4 (RBE4) express many proper...
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The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Adhesive interactions between cells regulate tissue integrity as well as the process of inflammatory cell recruitment. Such intercellular interactions are regulated by adhesion receptors and can be homotypic, ...
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Assays for the Calcium Sensitivity of Desmosomes
Epithelial cells in vivo exist as confluent cell sheets, but this confluence is disrupted if the sheets are wounded, if the cells are undergoing morphogenesis, or if they are taking part in invasion and metast...
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In Vitro Neutrophil Transepithelial Migration
Polymorphonuclear leukocyte (PMN) transmigration into tissues is a highly regulated process and plays a central role in host defense. In inflammatory human diseases such as ulcerative colitis and Crohn’s disea...
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Collagen Gel Contraction Assay
Mucosal tissues undergo contraction and relaxation on a continuous basis. In its normal state, the pliable intestinal tract is characterized by a rhythmic pattern of contractions controlled by its intrinsic ne...
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Cell-Cell Interactions on Solid Matrices
Models to study molecular, biochemical, and functional responses in vitro generally incorporate an individual cell type or group of cells organized in a random fashion. Normal physiological responses in vivo r...
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Methods to Assess Tissue Permeability
An essential requirement for adequate organ performance is the formation of permeability barriers that separate and maintain compartments of distinctive structure. The endothelial cell lining of the vasculatur...
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The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions
Adhesive interactions of cells with blood vessel walls under flow conditions are critical to a variety of processes, including hemostasis, leukocyte trafficking, tumor metastasis, and atherosclerosis. We have ...
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Tight Junctions and Cell–Cell Interactions
Chronic inflammation in mucosal tissues can influence epithelial barrier function via proinflammatory cytokines such as interferon (IFN)-γ and tumor necrosis factor-α. Increased mucosal levels of these cytokin...
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Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein α-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function
Disrupted epithelial cell junctions are a hallmark of numerous disease processes. The signaling mechanisms regulating barrier function and re-establishment of intact junctions after injury and during developme...
