11 Result(s)
within
Protocol
**aochen **
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Protocol
Measuring Protein Nuclear Transport via a Dual Reporter in iPSC-Derived Neurons
Nucleocytoplasmic transport (NCT) plays a critical role in maintaining cellular homeostasis, and its dysregulation has been shown to be implicated in many neurological diseases. To identify NCT deficits and de...
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Protocol
Nuclear mRNA Export Measurement Using FISH in iPSC-Derived Neurons
Nucleocytoplasmic transport (NCT) across the nuclear envelope plays a critical role in maintaining cellular homeostasis. Impaired NCT has been shown to be implicated in aging and many neurodegenerative disease...
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Protocol
Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration
Cell type-specific labelling and manipulation using Cre-driver lines have become integral to analyses of neuronal circuits in the brain. To study how mitral cells of the olfactory bulb process olfactory inform...
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Protocol
Semisynthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansion
Gln methylation is a newly identified histone mark and mediates ribosomal biogenesis. Site-specifically Gln-methylated proteins are valuable tools to elucidate the biological implications of this modification....
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Protocol
CRISPR-Based Transcriptional Activation in Drosophila
Overexpression is one of the classical approaches to study pleiotropic functions of genes of interest. To achieve overexpression, we often increase the transcription by introducing genes on exogenous vectors o...
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Protocol
Bioprinting of Complex Vascularized Tissues
Functional vasculature is crucial for the maintenance of living tissues via the transport of oxygen, nutrients, and metabolic waste products. As a result, insufficient vascularization in thick engineered tissu...
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Protocol
Gene Editing of Mouse Embryonic and Epiblast Stem Cells
Efficient and reliable methods for gene editing are critical for the generation of loss-of-gene function stem cells and genetically modified mice. Here, we outline the application of CRISPR-Cas9 technology for...
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Protocol
Using the Whole Cell Patch Clamp Technique to Study the Effect of Nanoparticles in Hippocampal Neurons
Several types of nanoparticles are considered toxic to the central nervous system. Patch-clamp is one of the most indispensable techniques in the study of neuroscience, especially in the field of neurophysiolo...
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Protocol
Dissecting Phagocytic Removal of Apoptotic Cells in Caenorhabditis elegans
The unique features of programmed cell death during C. elegans development provide an outstanding system to decipher the mechanisms governing phagocytic removal of apoptotic cells. Like in many other organisms, p...
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Protocol
Integration and Expression of gfp in the Plastid of Medicago sativa L.
Here we describe a protocol of alfalfa (Medicago sativa L.) plastid transformation by which gfp, a gene encoding the green fluorescent protein (GFP), is inserted into plastid genome via particle bombardment and h...
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Protocol
Projection Printing of 3-Dimensional Tissue Scaffolds
Our ability to create precise, predesigned, spatially patterned biochemical and physical microenvironments inside polymer scaffolds could provide a powerful tool in studying progenitor cell behavior and differ...
