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Protocol
Genetic Studies of Tic Disorders and Tourette Syndrome
Tourette syndrome (TS) is a complex disorder characterized by repetitive, sudden, and involuntary movements or vocalizations, called tics. Tics usually appear in childhood, and their severity varies over time....
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Protocol
Microiontophoretic Study of Individual Neurons During Intracellular Recording
There is an increasing tendency to combine multidisciplinary methods in studying the central nervous systems. One recently developed technique is the combination of electrophysiological and pharmacological inv...
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Protocol
Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots
This protocol describes a sensitive and rigorous method to monitor the movement and turnover of single synaptic vesicles in live presynaptic terminals of mammalian central nervous system. This technique makes ...
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Protocol
Converging Methodologies in a Mammalian “Simple System” Focused on the Biology of Memory: Conditioned Odor Preference in the Neonate Rat
We present here a critical period model for odor preference learning in the rat pup, which can produce short-term or lifelong changes in odor-related behaviors depending on training parameters. Features which ...
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Protocol
Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS
Grafting neural stem cells is a widely used experimental approach to central nervous system (CNS) repair after trauma or neurodegeneration. It is likely to be a realistic clinical therapy for human CNS disorde...
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Protocol
Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS
Stem cells have unlimited therapeutic potential for restoring CNS function lost secondary to trauma or degenerative disease. To date, stem cell grafts have only in a few instances partially ameliorated functio...
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Protocol
Electrophysiological Studies on Receptors In Vitro
In the past 20 years that in vitro preparations of central nervous system (CNS) cells have been used to access excitable membrane properties with electrophysiological techniques, there have been remarkable cha...