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    Protocol

    Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS

    Stem cells have unlimited therapeutic potential for restoring CNS function lost secondary to trauma or degenerative disease. To date, stem cell grafts have only in a few instances partially ameliorated functio...

    Qi-lin Cao, Stephen M. Onifer… in Neural Stem Cells: Methods and Protocols (2002)

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    Protocol

    Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS

    Grafting neural stem cells is a widely used experimental approach to central nervous system (CNS) repair after trauma or neurodegeneration. It is likely to be a realistic clinical therapy for human CNS disorde...

    Qi-lin Cao MD, PhD, Stephen M. Onifer PhD, Scott R. Whittemore PhD in Neural Stem Cells (2008)

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    Protocol

    Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots

    This protocol describes a sensitive and rigorous method to monitor the movement and turnover of single synaptic vesicles in live presynaptic terminals of mammalian central nervous system. This technique makes ...

    Qi Zhang in Chemical Neurobiology (2013)

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    Protocol

    The Role of Opioid Receptors in Migration and Wound Recovery In Vitro in Cultured Human Keratinocytes and Fibroblasts

    We have previously described significant changes in skin differentiation and the delay in wound healing from delta-opioid receptor knockout mice. In addition, we have shown that opioid receptor ligands and the...

    Mei Bigliardi-Qi, Paul L. Bigliardi in Opioid Receptors (2015)