Skip to main content

and
  1. No Access

    Protocol

    Reconstructing Signaling Networks Using Biosensor Barcoding

    Understanding how signaling networks are regulated offers valuable insights into how cells and organisms react to internal and external stimuli and is crucial for develo** novel strategies to treat diseases....

    Suyang Wang, Wei-Yu Chi, Gabriel Au, Cheng-Chieh Huang in Imaging Cell Signaling (2024)

  2. No Access

    Protocol

    MeDIP-on-Chip for Methylation Profiling

    DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlati...

    Yaw-Wen Hsu, Rui-Lan Huang, Hung-Cheng Lai M.D., Ph.D. in Cervical Cancer (2015)

  3. No Access

    Protocol

    A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template

    The hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase is essential for viral replication and a clinically validated antiviral target. Discovery of HCV polymerase inhibitors is greatly facilitated by th...

    Eric Ferrari, Hsueh-Cheng Huang in Antiviral Methods and Protocols (2013)

  4. No Access

    Protocol

    mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries

    mRNA display is a powerful in vitro selection technique that can be applied toward the identification of peptides or proteins with desired properties. The physical conjugation between a protein and its own RNA...

    Steve W. Cotten, Jianwei Zou, Rong Wang in Ribosome Display and Related Technologies (2012)

  5. No Access

    Protocol

    Methods for Rice Phenomics Studies

    With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene function. A systematic phenotypic profiling of mutant collections will provide major...

    Chyr-Guan Chern, Ming-Jen Fan, Sheng-Chung Huang, Su-May Yu in Plant Reverse Genetics (2011)

  6. No Access

    Protocol

    Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors

    Cell–cell interactions mediated by cell surface receptor–ligand pairs in the immune system are often of low affinity and transient in nature. To begin to study these weak interactions, it is desirable to devis...

    Hsi-Hsien Lin, Gin-Wen Chang, Yi-Shu Huang in Macrophages and Dendritic Cells (2009)

  7. No Access

    Protocol

    Bioinformatics

    The human genome project has opened up a new page in scientific history. To this end, a variety of techniques such as microarray has evolved to monitor the transcript abundance for all of the organism’s genes ...

    Hsueh-Fen Juan, Hsuan-Cheng Huang in Microarrays (2007)