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Protocol
Reconstructing Signaling Networks Using Biosensor Barcoding
Understanding how signaling networks are regulated offers valuable insights into how cells and organisms react to internal and external stimuli and is crucial for develo** novel strategies to treat diseases....
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Protocol
MeDIP-on-Chip for Methylation Profiling
DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlati...
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Protocol
A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template
The hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase is essential for viral replication and a clinically validated antiviral target. Discovery of HCV polymerase inhibitors is greatly facilitated by th...
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Protocol
mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries
mRNA display is a powerful in vitro selection technique that can be applied toward the identification of peptides or proteins with desired properties. The physical conjugation between a protein and its own RNA...
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Protocol
Methods for Rice Phenomics Studies
With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene function. A systematic phenotypic profiling of mutant collections will provide major...
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Protocol
Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors
Cell–cell interactions mediated by cell surface receptor–ligand pairs in the immune system are often of low affinity and transient in nature. To begin to study these weak interactions, it is desirable to devis...
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Protocol
Bioinformatics
The human genome project has opened up a new page in scientific history. To this end, a variety of techniques such as microarray has evolved to monitor the transcript abundance for all of the organism’s genes ...