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Protocol
Single-Cell RNA-Seq Technologies and Computational Analysis Tools: Application in Cancer Research
The recent maturation of single-cell RNA sequencing (scRNA-seq) provides unique opportunities for researchers to uncover new and potentially unexpected biological discoveries and to understand the complexity o...
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Protocol
Airway Inflammation Biomarker for Precise Management of Neutrophil-Predominant COPD
Chronic obstructive pulmonary disease (COPD) course can be divided into stable stage and acute exacerbation. Deepen the understanding to the function and role of airway inflammatory cells in stable COPD is imp...
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Protocol
Assays to Assess Arenaviral Glycoprotein Function
Arenaviruses, such as Lassa virus (LASV) and Pichindé virus (PICV), are enveloped viruses with a bi-segmented ambisense RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-depe...
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Protocol
Manufacturing and Delivering Genome-Editing Proteins
Genome-editing technologies have revolutionized the biomedical sciences by providing researchers with the ability to quickly and efficiently modify genes. While programmable nucleases can be introduced into ce...
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Protocol
Renal Delivery of Anti-microRNA Oligonucleotides in Rats
MicroRNAs are endogenous small, non-protein-coding RNA molecules that play an important role in the regulation of a wide variety of cellular functions and disease processes. A novel role for microRNAs in the d...
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Protocol
Tangled Trees: The Challenge of Inferring Species Trees from Coalescent and Noncoalescent Genes
Phylogenies based on different genes can produce conflicting phylogenies; methods that resolve such ambiguities are becoming more popular, and offer a number of advantages for phylogenetic analysis. We review ...