42 Result(s)
within
Protocol
**** Chu
Life Sciences
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Protocol
Imaging Cell Signaling in Tissues Using the IBEX Method
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial distribution of numerous pathways of interest simultaneously. Addit...
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Methods to Study Liver Disease Using Zebrafish Larvae
Liver disease affects millions of people worldwide, and the high morbidity and mortality is attributed in part to the paucity of treatment options. In many cases, liver injury self-resolves due to the remarkab...
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Defining Bacterial RNA-RNA Interactomes Using CLASH
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen accounting for high mortality rates among infected patients. Transcriptomic regulation by small RNAs (sRNAs) has been shown to regulate n...
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Map** Transcription Regulation with Run-on and Sequencing Data Using the Web-Based tfTarget Gateway
Run-on and sequencing assays like GRO-seq, PRO-seq, and ChRO-seq allow for joint profiling of transcription activity of transcriptional regulatory elements (TREs), i.e., promoters and active enhancers, and tar...
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Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates
Biomolecular condensates of ribonucleoproteins (RNPs) such as the transactivation response element (TAR) DNA-binding protein 43 (TDP-43) arise from liquid-liquid phase separation (LLPS) and play vital roles in...
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Correction to: Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
This chapter was inadvertently published with an error, the ninth point under ‘Materials’ section must read:
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Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes
Various types of stem cells and nonstem cells have been shown to differentiate or transdifferentiate into cardiomyocytes by way of coculture with appropriate inducer cells. Here we describe a method to induce ...
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DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles
A key factor for productive DNA-encoded libraries is the chemical diversity of the small molecule moiety attached to an encoding DNA oligomer. The library structure diversity is often limited to DNA-compatible...
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Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
Human pluripotent stem cells (hPSCs), such as induced pluripotent stem cells (iPSCs), hold great promise for drug discovery, toxicology studies, and regenerative medicine. Here, we describe standardized protoc...
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Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers
Genetic balancer systems, which allow effective capture and maintenance of lethal mutations stably, play an important role in identifying essential genes. Whole-genome sequencing (WGS) followed by bioinformati...
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FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs
With the development of fluorescent proteins (FPs) and advanced optical microscopy techniques, Förster or fluorescence resonance energy transfer (FRET) has become a powerful tool for real-time noninvasive visu...
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Molecular Simulations of Intrinsically Disordered Proteins and Their Binding Mechanisms
Intrinsically disordered proteins (IDPs) lack well-defined secondary or tertiary structures in solution but are found to be involved in a wide range of critical cellular processes that highlight their function...
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Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development
Virus-like particle (VLP) technology is an alternative platform for develo** vaccines to combat seasonal and pandemic influenza. Influenza VLPs are non-infectious nanoparticles that can elicit effective vacc...
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Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays
Microfluidic strategies combined with transduction and electronic integration have the promise of enabling miniaturized, combinatorial assays at higher speeds and lower costs, while at the same time mimicking ...
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Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell
Here, we report our step-by-step protocol for superparamagnetic nanoparticle (SPMNP)-based endosome and lysosome isolation from HeLa. Briefly, we synthesized SPMNP 1.0 with iron oxide (Fe3O4) core using thermal d...
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Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell
Here, we elaborate our detailed protocol for synthesis, functionalization, and application of superparamagnetic nanoparticle (SPMNP) for plasma membrane and lysosome isolation. We used standard thermal decompo...
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Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
Identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (BR) signaling. Establishment of methods to test for the hormone sensitivity is e...
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SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis
Dynamic tyrosine phosphorylation is a key molecular modulation for many signal transduction events. Because of their low abundance and dynamic nature in cells, the detection and enrichment of phosphotyrosine p...
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Pathway-Informed Discovery and Targeted Proteomic Workflows Using Mass Spectrometry
Recent advancements in mass spectrometry (MS) and data analysis software have enabled new strategies for biological discovery using proteomics. Proteomics has evolved from routine discovery and identification ...
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Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using b...
