24 Result(s)
within
Chapter
Lu**g Li
Biotechnology
Cell Biology
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Chapter
Fundamentals and Applications of Optically Active Melanin-Based Materials
Melanin is a group of ubiquitous biological pigments. It has excellent photonic properties such as broadband light absorption and high refractive index, combined with other physicochemical functions like free ...
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The Application of DNA Nanostructures in Vaccine Technology
Vaccine is a biological agent for preventing and curing disease, which inducts both innate and adaptive immune mechanism to be effective. Facing potentially unknown pathogens, the current vaccine technologies ...
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Bioimage Informatics for Big Data
Bioimage informatics is a field wherein high-throughput image informatics methods are used to solve challenging scientific problems related to biology and medicine. When the image datasets become larger and mo...
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Induced Pluripotent Stem Cells: Basics and the Application in Disease Model and Regenerative Medicine
Since the advent of induced pluripotent stem (iPS) cells at 2006, a flood of researches have been performed to study the application of iPS-based approaches in generating various types of disease models, perso...
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Current Status of Induced Pluripotent Stem Cells
The discovery of induced pluripotent stem cells (iPSCs) has “spiced up” the stem cell research field in the last few years. It has made tremendous progress in a very short time by demonstrating that adult fibr...
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Chapter and Conference Paper
Anti-Diabetes Effects of Hita Tenryou-Sui Water ® , a Natural Reduced Water
It has been reported that a natural reduced water derived from a deep well in Japan (trademark, Hita Tenryousui water®; Nakanoshima, Hita city, Oita, Japan) scavenged intracellular reactive oxygen species (ROS) a...
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Chapter and Conference Paper
Monoclonal Antibody Productivity of Hybridoma Cells Under Active Hypothermic Growth Condition
In our previous study, the enhanced specific monoclonal antibody (mAb) productivity of hybridoma cells grown under mild hypothermic condition (32 °C) was proportional to the population of S-phase cells, and in...
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Actin-based Chromosome Movements in Cell Division
Although microtubules are well-studied players moving chromosomes during mitosis and meiosis, recent work in mammalian oocytes has revealed intricate interactions between actin and chromosomes that directly co...
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Formin-Mediated Actin Assembly
Formins are remarkable large multi-domain proteins that utilize a novel mechanism to rapidly assemble actin filaments for diverse cellular processes such as division, motility, polarity, stress fibers and foca...
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Chapter
High-Throughput Screening Using Caco-2 Cell and PAMPA Systems
This chapter will focus on the two most commonly used high-throughput screening methods detecting cellular/bio-membrane permeability in the pharmaceutical industry: Caco-2 cells and the parallel artificial mem...
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Chapter and Conference Paper
Cotton 14-3-3L Gene Is Preferentially Expressed in Fiber
14-3-3 protein was originally identified during a systematic classification of bovine brain proteins. Thereafter, a number of 14-3-3 proteins have been found in every eukaryotic organism such as animal, fungi,...
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Chapter and Conference Paper
Inositol Polyphosphate 6-/3-kinase (AtIpk2β), an Early Auxin-Responsive Gene, Positively Regulates Axillary Shoot Branching in Arabidopsis thaliana
Arabidopsis inositol polyphosphate 6-/3-kinase gene (AtIpk2β) has previously been demonstrated to participate in inositol phosphate metabolism. However, overall little is known about its physiological functions i...
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Chapter and Conference Paper
An Arabidopsis Nudix Hydrolase Modulates the Innate Immune Response Independent of NPR1 and Salicylate
Plants have evolved complicated systems to regulate immune responses. The innate immune response which is triggered following recognition of conserved microbe-associated molecular patterns (MAMPs) provides the...
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Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls
Many questions in cell biology and biophysics involve the quantitation of the colocalisation of proteins tagged with different fluorophores and their interaction. However, the incomplete separation of the diff...
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Chapter
Rice
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Chapter and Conference Paper
Increase in the insulin secretion of HIT-T15 cells: gap junctional intercellular communications enhanced by hyaluronic acid
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Chapter and Conference Paper
Generation of natural killer cells from serum-free expanded CD34+ cells isolated from human umbilical cord blood
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Chapter and Conference Paper
Influence of natural reduced water on relevant tests parameters and reactive oxygen species concentration in blood of 320 diabetes patients in the prospective observation procedure
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Chapter
High Density and Scale-up Cultivation of Recombinant Cho Cell Line and Hybridomas with Porous Microcarrier Cytopore
Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant CHO cell line CL-11G producing prourokinase and hybridomas producing prourokinase mono...
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Chapter
Modification of Hybridoma Cells Metabolism
Two different genetic modifications of hybridoma cells are studied. First, cells are transfected with the glutamine synthetase gene, and important modifications in their physiology can be observed, that are re...
