28 Result(s)
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Article
Zhihong Gao
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Article
Open AccessSelection of reliable reference genes for gene expression studies in peach using real-time PCR
RT-qPCR is a preferred method for rapid and reliable quantification of gene expression studies. Appropriate application of RT-qPCR in such studies requires the use of reference gene(s) as an internal control t...
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Open AccessAnalysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers
Expressed Sequence Tag (EST) has been a cost-effective tool in molecular biology and represents an abundant valuable resource for genome annotation, gene expression, and comparative genomics in plants.
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Does Puffery Deceive? An Empirical Investigation
This paper reports four experiments that investigated whether puffery in advertising deceives or not. The first study tested the effects of six levels of puffery on consumer perceptions of ad truthfulness and ...
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A Modified Protocol for RNA Extraction from Different Peach Tissues Suitable for Gene Isolation and Real-Time PCR Analysis
RNA extraction is the first step in the study of gene isolation and expression. However, it is difficult to extract high quantity and quality RNA from tissues containing large quantities of polysaccharides and...
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Open AccessHigh-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot
MicroRNAs (miRNAs) are a class of endogenous, small, non-coding RNAs that regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in high plants. However, the di...
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Identification and validation of potential conserved microRNAs and their targets in peach (Prunus persica)
MicroRNAs are a class of small, endogenous, non-coding RNA molecules that negatively regulate gene expression at the transcriptional or the post-transcriptional level. Although a large number of miRNAs have be...
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A newly identified TSHβ splice variant is involved in the pathology of Hashimoto’s thyroiditis
Thyrotropin (TSH) is a protein that plays a key role in the control of thyroid function. TSH consists of a common α-subunit and a unique β-subunit; the latter is responsible for hormone specificity. A novel splic...
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Sequence Analysis of New S-RNase and SFB alleles in Japanese Apricot (Prunus mume)
As with other self-incompatible Prunus species, cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) display the S-RNase-based gametophytic self-incompatibility system. In this study, S-genotypes of ten Jap...
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Genome-wide expression profiles of seasonal bud dormancy at four critical stages in Japanese apricot
Dormancy is one of the most important adaptive mechanisms developed by perennial plants. To reveal the comprehensive mechanism of seasonal bud dormancy at four critical stages in Japanese apricot (Prunus persica)...
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Genome-wide identification of the auxin response factor (ARF) gene family and expression analysis of its role associated with pistil development in Japanese apricot (Prunus mume Sieb. et Zucc)
Auxin has been widely implicated in various aspects of plant growth and development, including flower development. In order to further elucidate the role of auxin during flower development, especially on the p...
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Thin film silicon solar module encapsulation technology research
The encapsulation failure is a serious problem which leads to power degradation and life time reduction of silicon based thin film solar module. Therefore, the encapsulation material and related technology res...
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Pistil abortion in Japanese apricot (Prunus mume Sieb. et Zucc.): isolation and functional analysis of PmCCoAOMT gene
The abnormal pistils widely occur in Japanese apricot (Prunus mume Sieb. et Zucc) and seriously affect the fruit production. In this study, a CCoAOMT homologue, PmCCoAOMT, was cloned in Japanese apricot and the b...
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Open AccessHigh-density genetic map construction and quantitative trait loci analysis of the stony hard phenotype in peach based on restriction-site associated DNA sequencing
Peach (Prunus persica) is an important fruit crop that generally softens rapidly after harvest resulting in a short shelf-life. By contrast, stony hard (SH) peach fruit does not soften and hardly produces ethylen...
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Transcriptomes of Fruit Cavity Revealed by De Novo Sequence Analysis in Nai Plum (Prunus salicina)
Nai plum (Prunus salicina) is an important fruit crop in China having good taste and flavour. Cavity formation occurring during fruit development affects fruit quality. However, the molecular mechanism underlying...
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Open AccessGenome-wide discovery and characterization of flower development related long non-coding RNAs in Prunus mume
Long non-coding RNAs (lncRNAs) are transcripts more than 200 bp in length do not encode proteins. Up to the present, it has been reported that lncRNAs play an essential role in developmental processes through ...
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A tunable single-longitudinal-mode wedge Nd:YVO4 laser with a YVO4 wave-plate
We report a tunable single-longitudinal-mode laser based on a wedged Nd:YVO4 gain medium and a YVO4 wave-plate. By taking advantage of the natural birefringence of YVO4 and adopting different cutting methods, a w...
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miR169 and PmRGL2 synergistically regulate the NF-Y complex to activate dormancy release in Japanese apricot (Prunus mume Sieb. et Zucc.)
This study is the first to demonstrate that GA4-induced dormancy release is associated with the NF-Y complex, which interacts with gibberellin inhibitor RGL2 in Japanese apricot.
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Comprehensive transcriptome profiling to identify genes involved in pistil abortion of Japanese apricot
Flower development exists as a key period in the angiosperms life cycle and the proper development is considered with its reproductive success. Pistil abortion is one of the widely distributed aspects of berry...
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Genome-wide identification and expression analysis of the frigida domain gene family in Prunus mume (Prunus mume Sieb. et Zucc.)
Frigida genes play a key role in regulating flowering time in plants. In this study, we identified 12 PmFRL genes and named them according to their chromosomal positions. These genes are unevenly distributed on f...
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Association between epicardial adipose tissue density and characteristics of coronary plaques assessed by coronary computed tomographic angiography
To investigate the relationship between the epicardial adipose tissue density (EATD) and the coronary plaque components as assessed by coronary computed tomographic angiography (CCTA). The study cohort include...
