-
Article
Effect of antisense oligodeoxynucleotide targeted against NF-κB/P65 on cell proliferation and tumorigenesis of gastric cancer
To study the inhibitory effect of nuclear transcription factor-kappa B(NF-κB) antisense oligodeoxynucleotide(ASODN) on the growth and tumorgenesis of human gastric cancer. We synthesized and transfected the AS...
-
Article
The highly conserved negatively charged Glu141 and Asp145 of the G-protein-coupled receptor RXFP3 interact with the highly conserved positively charged arginine residues of relaxin-3
Relaxin-3 is a newly identified insulin/relaxin superfamily peptide that plays a putative role in the regulation of food intake and stress response by activating its cognate G-protein-coupled receptor RXFP3. R...
-
Article
Role of ZIC1 methylation in hepatocellular carcinoma and its clinical significance
Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies in humans, and its prognosis is generally poor even after surgery. The zinc finger of the cerebellum (ZIC1) gene is a novel tumor suppr...
-
Article
Secretory overexpression and isotopic labeling of the chimeric relaxin family peptide R3/I5 in Pichia pastoris
Relaxin family peptides are a group of peptide hormones with divergent biological functions. Mature relaxin family peptides are typically composed of two polypeptide chains with three disulfide linkages, rende...
-
Article
Open AccessThe structure of f(R)-brane model
Recently, a family of interesting analytical brane solutions were found in f(R) gravity with \(f(R)=R \,+\, \alpha R^2\) ...
-
Article
Novel bioluminescent receptor-binding assays for peptide hormones: using ghrelin as a model
Peptide hormones perform important biological functions by binding specific cell membrane receptors. For hormone–receptor interaction studies, receptor-binding assays are widely used. However, conventional rec...
-
Article
Application of the novel bioluminescent ligand–receptor binding assay to relaxin-RXFP1 system for interaction studies
Relaxin is a prototype of the relaxin family peptide hormones and plays important biological functions by binding and activating the G protein-coupled receptor RXFP1. To study their interactions, in the presen...
-
Article
Open AccessMechanism for insulin-like peptide 5 distinguishing the homologous relaxin family peptide receptor 3 and 4
The relaxin family peptides play a variety of biological functions by activating four G protein-coupled receptors, RXFP1–4. Among them, insulin-like peptide 5 (INSL5) and relaxin-3 share the highest sequence h...
-
Article
Identification of hydrophobic interactions between relaxin-3 and its receptor RXFP3: implication for a conformational change in the B-chain C-terminus during receptor binding
Relaxin-3 is an insulin/relaxin superfamily neuropeptide implicated in the regulation of food intake and stress response via activation of the G protein-coupled receptor RXFP3. Their electrostatic interactions...
-
Article
A novel BRET-based binding assay for interaction studies of relaxin family peptide receptor 3 with its ligands
Relaxin family peptide receptor 3 (RXFP3) is an A-class G protein-coupled receptor that is implicated in the regulation of food intake and stress response upon activation by its cognate agonist relaxin-3. To s...
-
Article
Open AccessDevelopment of a selective agonist for relaxin family peptide receptor 3
Relaxin family peptides perform a variety of biological functions by activating four G protein-coupled receptors, namely RXFP1–4. Among these receptors, RXFP3 lacks a specific natural or synthetic agonist at p...
-
Article
Rapid preparation of bioluminescent tracers for relaxin family peptides using sortase-catalysed ligation
Relaxin family is a group of peptide hormones with a variety of biological functions by activating G protein-coupled receptors RXFP1–4. We recently developed bioluminescent tracers for their receptor-binding a...
-
Article
Development of a novel ligand binding assay for relaxin family peptide receptor 3 and 4 using NanoLuc complementation
Relaxin family peptides perform a variety of biological functions by binding and activating relaxin family peptide receptor 1–4 (RXFP1–4), four A-class G protein-coupled receptors. In the present work, we deve...
-
Article
Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5
The insulin superfamily is a group of homologous proteins that are further divided into the insulin family and relaxin family according to their distinct receptors. All insulin superfamily members contain thre...
-
Article
LEAP2 has antagonized the ghrelin receptor GHSR1a since its emergence in ancient fish
Recent studies have demonstrated that liver-expressed antimicrobial peptide 2 (LEAP2) antagonizes the ghrelin receptor GHSR1a in mammals. However, its antagonistic function in lower vertebrates has not yet bee...
-
Article
Nanomolar range of FAM237B can activate receptor GPR83
Our recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist...