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Article
Open AccessLong first exons and epigenetic marks distinguish conserved pachytene piRNA clusters from other mammalian genes
In the male germ cells of placental mammals, 26–30-nt-long PIWI-interacting RNAs (piRNAs) emerge when spermatocytes enter the pachytene phase of meiosis. In mice, pachytene piRNAs derive from ~100 discrete aut...
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Article
Open AccessStrand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abu...