8 Result(s)
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Article
William E. Theurkauf
English
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Article
Open AccessLong first exons and epigenetic marks distinguish conserved pachytene piRNA clusters from other mammalian genes
In the male germ cells of placental mammals, 26–30-nt-long PIWI-interacting RNAs (piRNAs) emerge when spermatocytes enter the pachytene phase of meiosis. In mice, pachytene piRNAs derive from ~100 discrete aut...
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Article
Open AccessStrand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abu...
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Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posterior
Microtubules and the plus-end-directed microtubule motor Kinesin I are required for the selective accumulation of oskar mRNA at the posterior cortex of the Drosophila melanogaster oocyte, which is essential to po...
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TACCing down the spindle poles
Centrosomes define the poles of mitotic spindles, but spindles that lack centrosomes mediate meiotic chromosome segregation in females. Recent studies provide new insight into anastral-spindle assembly, and in...
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Centrosomes and the Scrambled protein coordinate microtubule-independent actin reorganization
In Drosophila syncytial blastoderm embryos, centrosomes specify the position of actin-based interphase caps and mitotic furrows. Mutations in the scrambled locus prevent assembly of mitotic furrows, but do not bl...
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DNA-replication/DNA-damage-dependent centrosome inactivation in Drosophila embryos
During early embryogenesis of Drosophila melanogaster, mutations in the DNA-replication checkpoint lead to chromosome-segregation failures. Here we show that these segregation failures are associated with the ass...
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DNA-replication checkpoint control at the Drosophila midblastula transition
Embryogenesis is typically initiated by a series of rapid mitotic divisions that are under maternal genetic control1. The switch to zygotic control of embryogenesis at the midblastula transition is accompanied by...
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Article
Mechanical basis of meiotic metaphase arrest
CONTROL of the metaphase to anaphase transition is a central component of cell-cycle regulation. Arrest at either metaphase I or II before fertilization is a common component of oogenesis in many organisms1. In D...
