10 Result(s)
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Article
Shinsuke Ohnuki
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Article
Open AccessA microfluidic device to acquire high-magnification microphotographs of yeast cells
Yeast cell morphology was investigated to reveal the molecular mechanisms of cell morphogenesis and to identify key factors of other processes such as cell cycle progression. We recently developed a semi-autom...
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Open AccessSingle-cell phenomics reveals intra-species variation of phenotypic noise in yeast
Most quantitative measures of phenotypic traits represent macroscopic contributions of large numbers of cells. Yet, cells of a tissue do not behave similarly, and molecular studies on several organisms have sh...
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Profilin is required for Ca2+ homeostasis and Ca2+-modulated bud formation in yeast
A cls5-1 mutant of Saccharomyces cerevisiae is specifically sensitive to high concentrations of Ca2+, with elevated intracellular calcium content and altered cell morphology in the presence of 100 mM Ca2+. To rev...
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Open AccessUnveiling nonessential gene deletions that confer significant morphological phenotypes beyond natural yeast strains
Phenotypes are variable within species, with high phenotypic variation in the fitness and cell morphology of natural yeast strains due to genetic variation. A gene deletion collection of yeast laboratory strai...
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Open AccessGlobal study of holistic morphological effectors in the budding yeast Saccharomyces cerevisiae
The size of the phenotypic effect of a gene has been thoroughly investigated in terms of fitness and specific morphological traits in the budding yeast Saccharomyces cerevisiae, but little is known about gross mo...
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Open AccessCarotenoid dynamics and lipid droplet containing astaxanthin in response to light in the green alga Haematococcus pluvialis
The unicellular green alga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin to protect against environmental stresses. Haematococcus cells that accumulate astaxanthin in the...
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Implications of maintenance of mother–bud neck size in diverse vital processes of Saccharomyces cerevisiae
The mother–bud neck is defined as the boundary between the mother cell and bud in budding microorganisms, wherein sequential morphological events occur throughout the cell cycle. This study was designed to qua...
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Open AccessVirtual-freezing fluorescence imaging flow cytometry
By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology...
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Open AccessHigh-throughput platform for yeast morphological profiling predicts the targets of bioactive compounds
Morphological profiling is an omics-based approach for predicting intracellular targets of chemical compounds in which the dose-dependent morphological changes induced by the compound are systematically compar...
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Open AccessAssignment of unimodal probability distribution models for quantitative morphological phenoty**
Cell morphology is a complex and integrative readout, and therefore, an attractive measurement for assessing the effects of genetic and chemical perturbations to cells. Microscopic images provide rich informat...
