19 Result(s)
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Article
Sandro Keller
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A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca2+ and Mg2+ binding to EDTA
A small-scale ITC benchmarking study was performed involving 9 biophysics laboratories/facilities, to evaluate inter-laboratory and intra-laboratory basal levels of uncertainty. Our prime goal was to assess a ...
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Open AccessThe Molten Globule State of Maltose-Binding Protein: Structural and Thermodynamic Characterization by EPR Spectroscopy and Isothermal Titration Calorimetry
Employing site-directed spin labeling (SDSL), the structure of maltose-binding protein (MBP) had previously been studied in the native state by electron paramagnetic resonance (EPR) spectroscopy. Several spin-...
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Open AccessVIPP1 rods engulf membranes containing phosphatidylinositol phosphates
In cyanobacteria and plants, VIPP1 plays crucial roles in the biogenesis and repair of thylakoid membrane protein complexes and in co** with chloroplast membrane stress. In chloroplasts, VIPP1 localizes in d...
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Open AccessA minimal helical-hairpin motif provides molecular-level insights into misfolding and pharmacological rescue of CFTR
Our meagre understanding of CFTR misfolding and its reversal by small-molecule correctors hampers the development of mechanism-based therapies of cystic fibrosis. Here we exploit a helical-hairpin construct—th...
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Aggregation Behavior of pHLIP in Aqueous Solution at Low Concentrations: A Fluorescence Study
pH (low) insertion peptide (pHLIP) is a 36-residue peptide derived from the third transmembrane helix of the membrane protein bacteriorhodopsin. The hydrophobicity of this peptide makes it prone to aggregation...
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Lipid Membranes and Reactions at Lipid Interfaces: Theory, Experiments, and Applications
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Role of Coulombic Repulsion in Collisional Lipid Transfer Among SMA(2:1)-Bounded Nanodiscs
Styrene/maleic acid (SMA) and related copolymers are attracting great interest because they solubilise membrane proteins and lipids to form polymer-encapsulated nanodiscs. These nanodiscs retain a lipid-bilaye...
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Open AccessThermodynamics of nanodisc formation mediated by styrene/maleic acid (2:1) copolymer
Styrene/maleic acid copolymers (SMA) have recently attracted great interest for in vitro studies of membrane proteins, as they self-insert into and fragment biological membranes to form polymer-bounded nanodiscs ...
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Open AccessStructural and molecular comparison of bacterial and eukaryotic trigger factors
A considerably small fraction of approximately 60–100 proteins of all chloroplast proteins are encoded by the plastid genome. Many of these proteins are major subunits of complexes with central functions withi...
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Open AccessFast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs
Some styrene/maleic acid (SMA) copolymers solubilise membrane lipids and proteins to form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs preserve a lipid-bilayer core, they appe...
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Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions
This protocol from Brautigam et al. describes methods for baseline correction and global analysis of isothermal titration calorimetry data using NITPIC and SEDPHAT. Publication-quality graphs of resulting data ca...
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Journal of Membrane Biology: Biophysics
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Fluorierte Tenside und native Nano discs für die Membranproteinforschung
Integral membrane proteins are important but challenging research subjects. Various membrane mimetics have been developed that should preserve the native structure and function of membrane proteins while rende...
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Open AccessCharacterising protein/detergent complexes by triple-detection size-exclusion chromatography
In vitro investigations of membrane proteins usually depend on detergents for protein solubilisation and stabilisation. The amount of detergent bound to a membrane protein is relevant to successful experiment ...
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Modulating bilayer mechanical properties to promote the coupled folding and insertion of an integral membrane protein
Bilayer mechanical properties are not only of crucial importance to the mechanism of action of mechanosensation in lipid membranes but also affect preparative laboratory tasks such as membrane-protein refoldin...
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Protein folding in membranes
Separation of cells and organelles by bilayer membranes is a fundamental principle of life. Cellular membranes contain a baffling variety of proteins, which fulfil vital functions as receptors and signal trans...
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Nonlinear least-squares data fitting in Excel spreadsheets
We describe an intuitive and rapid procedure for analyzing experimental data by nonlinear least-squares fitting (NLSF) in the most widely used spreadsheet program. Experimental data in x/y form and data calculate...
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Monitoring detergent-mediated solubilization and reconstitution of lipid membranes by isothermal titration calorimetry
The solubilization and reconstitution of biological or liposomal membranes by detergents and biomolecules with detergent-like properties play a major role for technical applications (e.g., the isolation of mem...
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Uptake and release protocol for assessing membrane binding and permeation by way of isothermal titration calorimetry
The activity of many biomolecules and drugs crucially depends on whether they bind to biological membranes and whether they translocate to the opposite lipid leaflet and trans aqueous compartment. A general strat...
