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  1. No Access

    Article

    A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca2+ and Mg2+ binding to EDTA

    A small-scale ITC benchmarking study was performed involving 9 biophysics laboratories/facilities, to evaluate inter-laboratory and intra-laboratory basal levels of uncertainty. Our prime goal was to assess a ...

    Adrian Velazquez-Campoy, Bárbara Claro, Olga Abian… in European Biophysics Journal (2021)

  2. Article

    Open Access

    A minimal helical-hairpin motif provides molecular-level insights into misfolding and pharmacological rescue of CFTR

    Our meagre understanding of CFTR misfolding and its reversal by small-molecule correctors hampers the development of mechanism-based therapies of cystic fibrosis. Here we exploit a helical-hairpin construct—th...

    Georg Krainer, Antoine Treff, Andreas Hartmann, Tracy A. Stone… in Communications Biology (2018)

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  3. Article

    Lipid Membranes and Reactions at Lipid Interfaces: Theory, Experiments, and Applications

    Ana-Nicoleta Bondar, Sandro Keller in The Journal of Membrane Biology (2018)

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  4. No Access

    Article

    Role of Coulombic Repulsion in Collisional Lipid Transfer Among SMA(2:1)-Bounded Nanodiscs

    Styrene/maleic acid (SMA) and related copolymers are attracting great interest because they solubilise membrane proteins and lipids to form polymer-encapsulated nanodiscs. These nanodiscs retain a lipid-bilaye...

    Anne Grethen, David Glueck, Sandro Keller in The Journal of Membrane Biology (2018)

  5. No Access

    Article

    Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions

    This protocol from Brautigam et al. describes methods for baseline correction and global analysis of isothermal titration calorimetry data using NITPIC and SEDPHAT. Publication-quality graphs of resulting data ca...

    Chad A Brautigam, Huaying Zhao, Carolyn Vargas, Sandro Keller… in Nature Protocols (2016)

  6. Article

    Journal of Membrane Biology: Biophysics

    Sandro Keller, Ana-Nicoleta Bondar, Jana Broecker… in The Journal of Membrane Biology (2016)

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  7. No Access

    Article

    Fluorierte Tenside und native Nano discs für die Membranproteinforschung

    Integral membrane proteins are important but challenging research subjects. Various membrane mimetics have been developed that should preserve the native structure and function of membrane proteins while rende...

    Carolyn Vargas, Erik Frotscher, Rodrigo Cuevas Arenas, Anne Grethen… in BIOspektrum (2016)

  8. No Access

    Article

    Modulating bilayer mechanical properties to promote the coupled folding and insertion of an integral membrane protein

    Bilayer mechanical properties are not only of crucial importance to the mechanism of action of mechanosensation in lipid membranes but also affect preparative laboratory tasks such as membrane-protein refoldin...

    Michaela Herrmann, Bartholomäus Danielczak, Martin Textor… in European Biophysics Journal (2015)

  9. No Access

    Article

    Protein folding in membranes

    Separation of cells and organelles by bilayer membranes is a fundamental principle of life. Cellular membranes contain a baffling variety of proteins, which fulfil vital functions as receptors and signal trans...

    Sebastian Fiedler, Jana Broecker, Sandro Keller in Cellular and Molecular Life Sciences (2010)

  10. No Access

    Article

    Nonlinear least-squares data fitting in Excel spreadsheets

    We describe an intuitive and rapid procedure for analyzing experimental data by nonlinear least-squares fitting (NLSF) in the most widely used spreadsheet program. Experimental data in x/y form and data calculate...

    Gerdi Kemmer, Sandro Keller in Nature Protocols (2010)

  11. No Access

    Article

    Monitoring detergent-mediated solubilization and reconstitution of lipid membranes by isothermal titration calorimetry

    The solubilization and reconstitution of biological or liposomal membranes by detergents and biomolecules with detergent-like properties play a major role for technical applications (e.g., the isolation of mem...

    Heiko Heerklotz, Alekos D Tsamaloukas, Sandro Keller in Nature Protocols (2009)

  12. No Access

    Article

    Uptake and release protocol for assessing membrane binding and permeation by way of isothermal titration calorimetry

    The activity of many biomolecules and drugs crucially depends on whether they bind to biological membranes and whether they translocate to the opposite lipid leaflet and trans aqueous compartment. A general strat...

    Alekos D Tsamaloukas, Sandro Keller, Heiko Heerklotz in Nature Protocols (2007)