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Cell Biology
Ri** Feng
Article
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Article
Influence of kinetin on the membrane permeability ofAllium cepa epidermal cells
Nous avons employé une méthode plasmométrique pour étudier a perméabilité de la membrane d'Allium cepa. Après avoir fait nager l'épiderme d'un oignon sur la solution de kinetin (2,5 mg/l) pendant 10 h, on a trouv...
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Histochemical demonstration of succinic dehydrogenase activity during sequential molar development in the Swiss albino mouse
Applying the ditetrazolium salt (Nitro-BT) method for succinic dehydrogenase, murine molar teeth were studied sequentially from the cap stage of development through the appositional stages of odontogenesis. Re...
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Mineralogical differences in populations ofThais lapillus Linné (Gastropoda: Prosobranchiata)
Auf Grund des Verhältnisses Calcit/Aragonit in den Schalen vonThais lapillus können die 5 Populationen aus den Gewässern von New England in 2 Gruppen (5.99–6.77 und 14.50–18.25) aufgeteilt werden, was mit den mor...
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Presence of adenosine triphosphate in tobacco (Nicotiana tobacum) tissue grown in nutrient medium containing various concentrations of kinetin
On a fait croître du tissue de la moelle du tabac (Nicotiana tobacum) dans l'agar nourrissant ou dans un liquide contenant des minéraux basique et des hormones végétales. La croissance du tissu médullaire se mont...
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Determination of L-ascorbic acid levels in culture medium: Concentrations in commercial media and maintenance of levels under conditions of organ culture
The method of Deutsch and Weeks was modified to provide a reliable and reasonably quick method for assaying the L-ascorbic acid content of culture medium. The modified method was used to determine the decay of...
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Endplates after esterase inactivationin vivo: correlation between esterase concentration, functional response and fine structure
Mouse sternomastoid muscles were incubated with diisopropylfluorophosphate (DFP)in vivo, and the time course of recovery was studied using histochemistry, EM autoradiography and physiology. We found that: (1) the...
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Lysine metabolism in the human and the monkey: Demonstration of pipecolic acid formation in the brain and other organs
Metabolism ofl-[U-14C]lysine was studied in the human autopsy tissues and the intact monkeys through intracerebroventricular and intravenous injections. The human tissues were more active in the metabolism ofl-[1...
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Effect of ascorbic acid on protein synthesis and collagen hydroxylation in continuous flow organ cultures of adult mouse periodontal tissues
A continuous flow organ culture system (CFCS) was used to determine the effect of ascorbic acid on the synthesis of collagen and noncollagenous protein by bone of the alveolar process and periodontal ligament ...
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Blood-brain barrier transport ofL-pipecolic acid in various rat brain regions
Blood-brain barrier transport ofL-[l-14C]pipecolic acid was studied in the rat by single intracarotid injection using3H2O as a diffusible internal standard. Brain uptake index (BUI) forL-[14C]pipecolic acid (0.03...
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Aligning amino acid sequences: Comparison of commonly used methods
We examined two extensive families of protein sequences using four different alignment schemes that employ various degrees of “weighting” in order to determine which approach is most sensitive in establishing ...
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Progressive sequence alignment as a prerequisitetto correct phylogenetic trees
A progressive alignment method is described that utilizes the Needleman and Wunsch pairwise alignment algorithm iteratively to achieve the multiple alignment of a set of protein sequences and to construct an e...
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Structure-binding relationship of quinuclidinyl benzilate analogs on N4TG1 neuroblastoma muscarinic receptors
By Scatchard plot analysis of [3H]QNB (quinuclidinyl benzilate) binding, there are 2×105 muscarinic sites/cell with aK d about 10 nM in N4TG1 neuroblastoma cells. We have now e...
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Studies on the sites expressing evolutionary changes in the structure of eukaryotic 5S ribosomal RNA
We have determined the complete sequences of 5S rRNAs from a lamprey (Lampetra reissneri), a lancelet (Branchiostoma belcheri), silkworms (Philosamia cynthia ricini, Bombyx mori, Antheraea pernyi), and a silkworm...
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Velocity distribution on the membrane of a tank-treading red blood cell
The kinematics of an area-conserving tank-treading disk-shaped red blood cell membrane is studied using the stream function method suggested by Secomb and Skalak (Q. Jl Mech. appl. Math. 3...
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The yeast Yarrowia lipolytica has two, functional, signal recognition particle 7S RNA genes
Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstra...
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Molecular cloning and expression of a proteinase gene from Lactococcus lactis subsp. cremoris H2 and construction of a new lactococcal vector pFX1
The 6.5 kb HindIII DNA fragment of the Lactococcus lactis subsp. cremoris H2 plasmid pDI21 was cloned into Escherichia coli POP13 with λNM1149, and also directly into Lactococcus lactis subsp. lactis 4125 using a...
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A naturally occurring horizontal gene transfer from a eukaryote to a prokaryote
Naturally occurring horizontal gene transfers between nonviral organisms are difficult to prove. Only with the availability of sequence data from a wide variety of organisms can a convincing case be made. In t...
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High frequency production of haploid embryos in asparagus anther culture
A method for obtaining a high frequency of haploid asparagus embryos through anther culture was developed. Flowers collected from plants in the field in July, August and September 1990, for the genotype G203, ...
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A tissue culture model for studying ethanol toxicity on embryonic heart cells
A tissue system in which fibroblasts and myocytes from chick embryonic hearts were separately maintained was used to study the toxicity of ethanol. To reproduce the teratogenic effects of acute, high concentra...
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In vitro plant regeneration of spinach from mature seed-derived callus
A system for the regeneration of spinach (Spinacia oleracea L.) from mature dry seed explants has been established. The response of two commercial spinach cultivars, ‘Grandstand’ and ‘Baker’, was examined. Callus...
