1,651 Result(s)
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Article
Lu**g Li
Biochemistry, general
Biotechnology
Cell Biology
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Article
Influence of glass microbeads on growth, activity and morphological changes of Bacillus megaterium
Cells of Bacillus megaterium growing in the presence of glass microbeads with average diameters of 29 and 53 μm were frequently filamentous and sometimes reached lengths of 600 μm. Some of the filaments were nons...
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Synchronization of 9L rat brain tumor cells by centrifugal elutriation
Asynchronous 9L cells were separated into relatively homogeneously-sized populations using centrifugal elutriation with both a conventional collection method and a long collection method. A substantial increas...
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Characterization of the separation properties of the beckman elutriator system
The role of fluid flow in the elutriation process was visualized by pum** dye solution through the Beckman JE-6 elutriator rotor. Three major fluid flow disturbances were observed in the separation chambers,...
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Biophoton emission
The phenomenon of ultraweak photon emission from living systems was further investigated in order to elucidate the physical properties of this radiation and its possible source. We obtained evidence that the l...
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Ammonia switch-off of nitrogenase from Rhodobacter sphaeroides and Methylosinus trichosporium: no evidence for Fe protein modification
In vivo switch-off of nitrogenase activity by NH 4 + is a reversible process in Rhodobacter sphaeroides and Methylosinus trichosporium OB3b. The same pattern of...
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Scavenging effect of extracts of green tea and natural antioxidants on active oxygen radicals
With the use of the spin trap** methods, the scavenging effects of the extracts of green tea and other natural foods are studied. In stimulated polymorphonuclear leukocytes (PMN) system, water extract fracti...
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Physiological characteristics of glutamine synthetases I and II of Frankia sp. strain CpI1
Frankia sp. strain CpI1 has two glutamine synthetases designated GSI and GSII. Biosynthetic activities of both GSI and GSII were strongly inhibited by ADP and AMP. Alanine, aspartate, glycine and serine inhibite...
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In vitro studies of deformation and adhesion properties of transformed cells
The micropipet aspiration technique and the parallel-plate flow chamber were used to investigate the deformation and detachment properties, respectively, of normal and transformed rat fibroblasts. The normal C...
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Animal models of human-derived cancer vaccines
Preclinical cancer vaccine studies must address vaccine safety, immunogenicity, and efficacy, as well as mechanism of vaccine action. Animal models of vaccines employing human tumor-associated antigen or epito...
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Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display
Methods for retrieving and reamplifying the differentially expressed cDNA bands have been modified. Direct reamplification of differentially expressed bands after cutting from a polyacrylamide gel (PAG) follow...
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Filamentous bacteriophage display of a bifunctional protein A::scFv fusion
Filamentous bacteriophage display is a powerful and widely used technology for the selection of affinity ligands. However, the commonly used phagemid systems result in the production of a population of phage o...
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Purification and physical characterization of cloned human cAMP phosphodiesterases PDE-4D and-4C
Individual isozymes of family four cyclic-nucleotide phosphodiesterases (PDE-4s) were characterized and compared in order to advance our understanding of how PDE-4s regulate cAMP levels in cells. Full-length a...
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Generation of stable recombinant retroviruses containing the β-globin genes linked to complex regulatory elements by using transient transfection
A transient packaging method for a retroviral vector containing β-globin sequences, mediated by Ca3(PO4)2 precipitation, generated stable recombinant β-globin retroviruses with intact viral genomes. We suggest th...
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Comparison of two kinds of methods to determine the titer of recombinant retrovirus containing β-globin gene based on G418 selection
Four recombinant retrovirus (RV) vectors containing human β-globin gene and regulatory elements were constructed. To determine the titers of recombinant RV from corresponding producer cell lines, we compared t...
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Cytoplasmic expression of a soluble synthetic mammalian metallothionein-α domain in Escherichia coli
Bacteria are commonly used for bioremediation of heavy metal pollution and strategies to improve their performance in this respect are desirable. In this study, an Escherichia coli strain was engineered to expres...
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The Myxococcus xanthus wbgB gene encodes a glycosyltransferase homologue required for lipopolysaccharide O-antigen biosynthesis
Myxococcus xanthus is a gram-negative soil bacterium that initiates a complex developmental program in response to starvation. A transposon insertion (Tn5-lac Ω109) mutant with developmental deficiencies was iso...
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Negative regulation of Janus kinases
The precise regulation of both the magnitude and the duration of Janus kinase (JAK) catalytic activity is essential for the cytokine orchestration of many biological processes, and the dysregulation of JAK act...
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Novel functional assay for proteases and modulators
Proteases play a critical role in many cellular functions and have been an attractive therapeutic target due to their involvement in a number of disease processes. One prominent example is the secretases respo...
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Analysis of the stress resistance of commercial wine yeast strains
Alcoholic fermentation is an essential step in wine production that is usually conducted by yeasts belonging to the species Saccharomyces cerevisiae. The ability to carry out vinification is largely influenced b...
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Pattern of cyanophycin accumulation in nitrogen-fixing and non-nitrogen-fixing cyanobacteria
The temporal and spatial accumulation of cyanophycin was studied in two unicellular strains of cyanobacteria, the diazotrophic Cyanothece sp. strain ATCC 51142 and the non-diazotrophic Synechocystis sp. strain P...
