241 Result(s)
within
English
Life Sciences
Li-**n Tao
Article
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Article
A method for isolating and culturing vascular smooth muscle cells from porcine cerebral arteries
Cerebral blood vessels have unique properties when compared to most peripheral vascular beds. One such property is that large cerebral vessels are involved in the regulation of cerebral vascular resistance. St...
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A new evolutionary algorithm for function optimization
A new algorithm based on genetic algorithm(GA) is developed for solving function optimization problems with inequality constraints. This algorithm has been used to a series of standard test problems and exhibi...
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Quantitative dynamics on stimulating regeneration and sowing seedlings ofLarix gmelinii in Daxing’an Mountains
To understand the quantitative dynamics and death reason of stimulating regeneration seedlings is significant for stimulating the natural regeneration ofLarix gmelinii and implement of conservation project of nat...
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Arbuscular mycorrhizal status of plants and the spore density of arbuscular mycorrhizal fungi in the tropical rain forest of **shuangbanna, southwest China
The arbuscular mycorrhizal status of 112 plant species and the spore density of arbuscular mycorrhizal fungi (AMF) in the rhizosphere soil of these plants in the tropical rain forest of **shuangbanna, southwe...
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Endogenous hormone levels at technical maturing stage of sugarcane
Ethylene evolution of leaf and contents of ABA and IAA in cane-tip were measured during late growth stage of sugarcane. The ethylene evolution of different leaves (the leaf with TVD was defined as leaf +1, oth...
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Tele-network design based on queue competition algorithm
In this paper, we report research on how to design the tele-network. First of all, we defined the reliability of tele-network. According to the definition, we divide the whole reliability into two parts: the r...
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Developmental analyses reveal early arrests of the spore-bearing parts of reproductive organs in unisexual flowers of cucumber (Cucumis sativus L.)
To understand the regulatory mechanisms governing unisexual flower development in cucumber, we conducted a systematic morphogenetic analysis of male and female flower development, examined the dynamic changes ...
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A recombination-based strategy for assembling open-reading frames
A recombination-based strategy was developed for the assemble of valid open-reading frames. Compared with the conventional reverse transcription-polymerase chain reaction (PCR) approach, this strategy eliminat...
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Differential gene expression during stationary phase between amicronucleates and micronucleates of the ciliated protist, Pseudourostyla cristata
Ciliates are unicellular eukaryotic organisms with two types of nuclei, the ‘germline’ micronucleus (MIC) and the ‘somatic’ macronucleus (MAC). We previously reported that when the MIC of Pseudourostyla cristata ...
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Identification of a new nitrogen fixing endo-bacterium strain isolated from sugarcane stalk
A bacterial strain B8S, with nitrogen fixation ability, was isolated from the stalk of sugarcane variety RB867515 introduced from Brazil. This strain was identified based on morphological, physiological and bi...
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Recombinant production of fibrinogenase IV from Agkistrodon acutus venom and its preliminary evaluation
A novel metalloproteinase, recombinant fibrinogenase IV (rFIVa), was expressed and purified from Agkistrodon acutus venom. It is a single-chain protein with an apparent molecular weight of 27 kDa. Western blot sh...
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Cloning of three members of acc synthase gene family in sugarcane
To clone the members of ACC synthase gene family in sugarcane, two degenerate oligonucleotide primers were designed, coding for two conservative acid regons in ACC synthase protein family. PCR amplification wa...
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Inhibitory effects of neoandrographolide on nitric oxide and prostaglandin E2 production in LPS-stimulated murine macrophage
Activated macrophages express inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), produce excessive amounts of nitric oxide (NO) and prostaglandin E2 (PGE2), which play key roles in t...
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Open AccessAnalysis of 142 genes resolves the rapid diversification of the rice genus
The completion of rice genome sequencing has made rice and its wild relatives an attractive system for biological studies. Despite great efforts, phylogenetic relationships among genome types and species in th...
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Molecular cloning and tissue specific expression of an elongation factor 1A gene in sugarcane stalks
Genomic reconstruction done by using the cDNA insert containing the ORF of GZ-1-alpha as the probe indicated the presence of about 5 copies of eEF1A gene per haploid sugarcane genome. Northern hybridization st...
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Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR
Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (m...
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Transformation of sugarcane with ACC oxidase antisense gene
The present study is an attempt to validate the function of sugarcane ACO by construction of plant expression vectors for sugarcane ACO sense and anti-sense genes and their effective transformation. Plant expr...
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Distribution, biomass, and dynamics of roots in a revegetated stand of Caragana korshinskii in the Tengger Desert, northwestern China
A field experiment was conducted to investigate root distribution, biomass, and seasonal dynamics in a revegetated stand of Caragana korshinskii Kom. in the Tengger Desert. We used soil profile trenches, soil cor...
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Overexpression of Thellungiella halophila H+-PPase (TsVP) in cotton enhances drought stress resistance of plants
An H+-PPase gene, TsVP from Thellungiella halophila, was transferred into two cotton (Gossypium hirsutum) varieties (Lumianyan19 and Lumianyan 21) and southern and northern blotting analysis showed the foreign ge...
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Mutation ofgyrA andparC in clinical isolates ofAcinetobacter baumannii and its relationship with antimicrobial drugs resistance in Taiwan
Acinetobacter baumannii is a species of non fermentative Gram-negative bacteria commonly found in water and soil. This organism was susceptible to most antibiotics in the 1970s. It has now become...
