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Article
Elements in erythrocytes of population with different thyroid hormone status
The contents of elements K, Ca, Fe, Cu, Zn, Se, and Rb in erythrocytes of 78 cases with different thyroid hormone status have been measured by proton-induced X-ray emission and neutron activation analysis. Acc...
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Article
Study of trace elements in blood of thyroid disorder subjects before and after 131I therapy
Both trace elements and thyroid hormones play essential roles in the human body. However, the previous results about the interaction between these two factors are often controversially given. In order to make ...
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Article
Establishment of Chinese soybean Glycine max core collections with agronomic traits and SSR markers
It is very important to efficiently study and use genetic diversity resources in crop breeding and sustainable agriculture. In this study, different sampling methods and sample sizes were compared in order to ...
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Article
Molecular cloning and characterization of a novel splice variant of the LIM domain family gene, PINCH 2, in human testis
By hybridizing human adult testis cDNA microarrays with human adult and embryo testis cDNA probes, we identified a novel human testis gene, PINCH 2. PINCH 2 expression was 3.4-fold higher in adult than in feta...
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Article
Effect of metabolic structures and energy requirements on curdlan production byAlcaligenes faecalis
A comprehensive metabolic network was proposed forAlcaligenes faecalis and employed in a stoichiometrically based flux balance model for curdlan production optimization. The maximal yield of curdlan was evaluated...
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Article
Genetic structure and diversity of cultivated soybean (Glycine max (L.) Merr.) landraces in China
The Chinese genebank contains 23,587 soybean landraces collected from 29 provinces. In this study, a representative collection of 1,863 landraces were assessed for genetic diversity and genetic differentiation...
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Article
Expressions of three cotton genes encoding the PIP proteins are regulated in root development and in response to stresses
Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters water stress such as drought or waterlog during its growth season (Summer). To investigate molecular mechanism of water reg...
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Article
Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis
Micromonospora inyoensis produces sisomicin (Sm), an aminoglycoside antibiotic. The gene cluster of sisomicin biosynthesis spanning ca. 47 kb consists of 37 ORFs encoding various proteins...
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Article
Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis
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Article
Curing the Plasmid pXO2 from Bacillus anthracis A16 Using Plasmid Incompatibility
Plasmid incompatibility, which has no effect on other plasmids or chromosomal genes, can be used to cure a target plasmid. In this report, we successfully cured the plasmid pXO2 from Bacillus anthracis A16 with a...
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Article
Effect of Silent Mutations in Translational Initial Region on the Production of Recombinant Cutinase in Escherichia coli
Translational Initial Region (TIR) is the threshold of an intracellular translation process, and tiny alterations in this region are reported to intensely influence the downstream expression. Such property pro...
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Article
Removal of l-alanine from the production of l-2-aminobutyric acid by introduction of alanine racemase and d-amino acid oxidase
l-2-Aminobutyric acid can be synthesized in a transamination reaction from l-threonine and l-aspartic acid as substrates by the action of threonine deaminase and aromatic aminotransferase, but the by-product l-al...
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Article
Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.)
In this study, one major QTL affecting gel consistency (GC) of japonica/indica cross was identified on chromosome 6 using a DH population. To understand the molecular mechanism that regulates GC in rice grains...
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Article
A Causal Analysis of Intra-Abdominal Hemorrhage After Reduced-Size Liver Transplantation in Rat
In this study, we analyzed causes of abdominal hemorrhage after reduced-size liver transplantation in rat. Healthy SD rats (weight range of 260–280 g) underwent liver transplantation, the donors were female an...
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Article
Acquiring transgenic tobacco plants with insect resistance and glyphosate tolerance by fusion gene transformation
The advantages of gene ‘stacking’ or ‘pyramiding’ are obvious in genetically modified (GM) crops, and several different multi-transgene-stacking methods are available. Using linker peptides for multiple gene t...
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Article
Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01)
ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Comple...
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Article
A new polysialic acid production process based on dual-stage pH control and fed-batch fermentation for higher yield and resulting high molecular weight product
To determine the factors influencing the resulting molecular weight of polysialic acid (PSA), batch fermentations by using Escherichia coli were conducted. It was found that temperature and pH were significant fa...
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Article
Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1
RNA interference (RNAi) is a biological phenomenon that silences the expression of genes of interest. Passive double-stranded RNA (dsRNA) uptake has been uniquely observed in Caenorhabditis elegans due to the exp...
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Article
Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects
The study of insect-resistant transgenic tobacco provides a good foundation for the further application of the cry1Ah gene in other important crops. ...
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Article
A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension
Biologically active β-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of curdlan polysaccharide. The water-insoluble curdlan impedes its hydrolysis efficiency whi...