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Article
Open AccessDisease-induced changes in bacterial and fungal communities from plant below- and aboveground compartments
The plant microbes are an integral part of the host and play fundamental roles in plant growth and health. There is evidence indicating that plants have the ability to attract beneficial microorganisms through...
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Article
Open AccessImproving the production of carbamoyltobramycin by an industrial Streptoalloteichus tenebrarius through metabolic engineering
Tobramycin is an essential and extensively used broad-spectrum aminoglycoside antibiotic obtained through alkaline hydrolysis of carbamoyltobramycin, one of the fermentation products of Streptoalloteichus tenebra...
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Article
Optimization of welan gum extraction and purification using lysozyme and alkaline protease
Welan gum, a natural polysaccharide produced by Sphingomonas sp. ATCC 31555, has attracted considerable attention in the scientific community due to its desirable properties. However, challenges, such as high vis...
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Article
Open AccessPreparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli
Engineered strains of Escherichia coli have been used to produce bioconjugate vaccines using Protein Glycan Coupling Technology (PGCT). Nanovaccines have also entered the vaccine development arena with advances i...
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Article
Open AccessManipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency
Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or mo...
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Article
Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis