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Article
Open AccessPreparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli
Engineered strains of Escherichia coli have been used to produce bioconjugate vaccines using Protein Glycan Coupling Technology (PGCT). Nanovaccines have also entered the vaccine development arena with advances i...
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Article
Open AccessManipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency
Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or mo...
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Article
Open AccessSoft - sensing modeling based on ABC - MLSSVM inversion for marine low - temperature alkaline protease MP fermentation process
Aiming at the characteristics of nonlinear, multi-parameter, strong coupling and difficulty in direct on-line measurement of key biological parameters of marine low-temperature protease fermentation process, a...