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    Article

    Enhanced N-acetyl-d-neuraminic production from glycerol and N-acetyl-d-glucosamine by metabolically engineered Escherichia coli with a two-stage pH-shift control strategy

    Typical N-acetyl-d-neuraminic acid (Neu5Ac) production uses N-acetyl-d-glucosamine (GlcNAc) and excess pyruvate as substrates in the enzymatic or whole-cell biocatalysis process. In a previous study, a Neu5Ac-pro...

    De-qiang Zhu, Jian-rong Wu, **ao-bei Zhan… in Journal of Industrial Microbiology & Biote… (2019)

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    Article

    Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system

    Yarrowia lipolytica is categorized as a generally recognized as safe (GRAS) organism and is a heavily documented, unconventional yeast that has been widely incorporated into multiple indu...

    Shuliang Gao, Yangyang Tong, Zhiqiang Wen… in Journal of Industrial Microbiology & Biote… (2016)