23 Result(s)
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Microbial Genetics and Genomics
Guo-**n Jiang
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Open AccessOral pathogens exacerbate Parkinson’s disease by promoting Th1 cell infiltration in mice
Parkinson’s disease (PD) is a common chronic neurological disorder with a high risk of disability and no cure. Periodontitis is an infectious bacterial disease occurring in periodontal supporting tissues. Stud...
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Open AccessSelection of functional EPHB2 genotypes from ENU mutated grass carp treated with GCRV
N-ethyl-N-nitrosourea (ENU) mutagenesis is a useful method for the genetic engineering of plants, and the production of functional mutants in animal models including mice and zebrafish. Grass carp reovirus (GCRV)...
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Open AccessA simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease
The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional purification approaches, including affinity purification, gel...
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Open AccessSoil pH is the primary factor driving the distribution and function of microorganisms in farmland soils in northeastern China
To understand which environmental factors influence the distribution and ecological functions of bacteria in agricultural soil.
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Open AccessIdentification of differentially expressed genes and pathways between intramuscular and abdominal fat-derived preadipocyte differentiation of chickens in vitro
The distribution and deposition of fat tissue in different parts of the body are the key factors affecting the carcass quality and meat flavour of chickens. Intramuscular fat (IMF) content is an important fact...
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Open AccessTranscriptional reference map of hormone responses in wheat spikes
Phytohormones are key regulators of plant growth, development, and signalling networks involved in responses to diverse biotic and abiotic stresses. Transcriptional reference maps of hormone responses have bee...
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Open AccessAntimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)
Phormicins belong to defensin family, which are important antimicrobial peptides (AMPs) in insects. These AMPs are inducible upon challenging by immune triggers. In the present study, we identified the cDNA of...
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Open AccessChromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Salvianic acid A (SAA), a valuable natural product from herbal plant Salvia miltiorrhiza, exhibits excellent antioxidant activities on food industries and efficacious therapeutic potential on cardiovascular disea...
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Open AccessEnhanced production of polyhydroxybutyrate by multiple dividing E. coli
Most bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and m...
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Open AccessGiniClust: detecting rare cell types from single-cell gene expression data with Gini index
High-throughput single-cell technologies have great potential to discover new cell types; however, it remains challenging to detect rare cell types that are distinct from a large population. We present a novel...
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Open AccessGenome-wide association study for flowering time, maturity dates and plant height in early maturing soybean (Glycine max) germplasm
Soybean (Glycine max) is a photoperiod-sensitive and self-pollinated species. Days to flowering (DTF) and maturity (DTM), duration of flowering-to-maturity (DFTM) and plant height (PH) are crucial for soybean ada...
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Open AccessGenome organisation of the Acinetobacter lytic phage ZZ1 and comparison with other T4-like Acinetobacter phages
Phage ZZ1, which efficiently infects pathogenic Acinetobacter baumannii strains, is the fifth completely sequenced T4-like Acinetobacter phage to date. To gain a better understanding of the genetic characteristic...
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Open AccessComplete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Amycolatopsis orientalis is the type species of the genus and its industrial strain HCCB10007, derived from ATCC 43491, has been used for large-scale production of the vital antibiotic vancomycin. However, to dat...
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Open AccessHigh throughput sequencing of two celery varieties small RNAs identifies microRNAs involved in temperature stress response
MicroRNAs (miRNAs) are small, non-coding RNAs of 20 to 24 nucleotides that regulate gene expression and responses to biotic and abiotic stress. Till now, no reports have previously been published concerning mi...
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Open AccessMetabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains
Shikimic acid (SA) is a key chiral starting molecule for the synthesis of the neuramidase inhibitor GS4104 against viral influenza. Microbial production of SA has been extensively investigated in Escherichia coli
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Open AccessGlobal analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients
Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are est...
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Open AccessHyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli
Poly(4-hydroxybutyrate) [poly(4HB)] is a strong thermoplastic biomaterial with remarkable mechanical properties, biocompatibility and biodegradability. However, it is generally synthesized when 4-hydroxybutyra...
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Genetic characteristics of 2009 pandemic H1N1 influenza a viruses isolated from Mainland China
A total of 100 H1N1 flu real-time-PCR positive throat swabs collected from fever patients in Zhejiang, Hubei and Guangdong between June and November 2009, were provided by local CDC laboratories. After MDCK ce...
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Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence
Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune and memory response via either DNA or protein vaccines. To improve immunogenicity, efforts have focused on high-leve...
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Open AccessIdentification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms
Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L
