12 Result(s)
within
Microbial Genetics and Genomics
Microbiology
Guo-**n Jiang
Article
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Article
Screening of a high growth influenza B virus strain in Vero cells
Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemerg...
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Open AccessIdentification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms
Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L
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Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence
Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune and memory response via either DNA or protein vaccines. To improve immunogenicity, efforts have focused on high-leve...
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Genetic characteristics of 2009 pandemic H1N1 influenza a viruses isolated from Mainland China
A total of 100 H1N1 flu real-time-PCR positive throat swabs collected from fever patients in Zhejiang, Hubei and Guangdong between June and November 2009, were provided by local CDC laboratories. After MDCK ce...
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Open AccessHyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli
Poly(4-hydroxybutyrate) [poly(4HB)] is a strong thermoplastic biomaterial with remarkable mechanical properties, biocompatibility and biodegradability. However, it is generally synthesized when 4-hydroxybutyra...
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Open AccessMetabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains
Shikimic acid (SA) is a key chiral starting molecule for the synthesis of the neuramidase inhibitor GS4104 against viral influenza. Microbial production of SA has been extensively investigated in Escherichia coli
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Open AccessEnhanced production of polyhydroxybutyrate by multiple dividing E. coli
Most bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and m...
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Open AccessChromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Salvianic acid A (SAA), a valuable natural product from herbal plant Salvia miltiorrhiza, exhibits excellent antioxidant activities on food industries and efficacious therapeutic potential on cardiovascular disea...
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Open AccessAntimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)
Phormicins belong to defensin family, which are important antimicrobial peptides (AMPs) in insects. These AMPs are inducible upon challenging by immune triggers. In the present study, we identified the cDNA of...
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Open AccessSoil pH is the primary factor driving the distribution and function of microorganisms in farmland soils in northeastern China
To understand which environmental factors influence the distribution and ecological functions of bacteria in agricultural soil.
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Open AccessA simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease
The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional purification approaches, including affinity purification, gel...
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Open AccessOral pathogens exacerbate Parkinson’s disease by promoting Th1 cell infiltration in mice
Parkinson’s disease (PD) is a common chronic neurological disorder with a high risk of disability and no cure. Periodontitis is an infectious bacterial disease occurring in periodontal supporting tissues. Stud...
