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Article
Construction and characterisation of a hexaploid wheat (Triticum aestivum L.) BAC library from the reference germplasm ‘Chinese Spring’
Hexaploid wheat (Triticum aestivum L.) is one of the most important crops in the world with 582, 691, 612 million tonnes produced in 2001 on one third of the world’s cultivated area. It has a very large genome (1...
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Article
A simple PCR-based method for scoring the ph1b deletion in wheat
The amplified fragment length polymorphism (AFLP) technique was used to isolate DNA sequences present in the euploid wheat Chinese Spring but not in the Chinese Spring ph1b mutant (which has a deletion of the Ph1
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Article
Flow cytometric analysis of the chromosomes and stability of a wheat cell-culture line
A rapidly growing, long-term suspension culture derived from Triticum aestivum L. (wheat) was synchronized using hydroxyurea and colchicine, and a chromosome suspension with 2–3 × 106 chromosomes ml−1 was made. A...
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Article
RFLP-based genetic maps of the homoeologous group 5 chromosomes of bread wheat (Triticum aestivum L.)
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Article
HpaII library indicates ‘methylation-free islands’ in wheat and barley
A library of wheat genomic DNA HpaII tiny fragments (HTF), sized below 500 bp, has been constructed. Of the clones in the library 80% belong to the single/low-copy category, while 12% of the clones are nuclear re...