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Open AccessGenetic diversity analysis and DNA fingerprinting of primary Qingke (Hordeum vulgare L. var. nudum Hook. f.) cultivars
To assess the genetic diversity of the primary Qingke cultivars and establish their unique genetic profiles, 837 barley simple sequence repeat (SSR) primers were screened across 12 cultivars. The selection pro...
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Article
Genome-wide assessment of genetic variation and differentiation for Gastrodia elata germplasm based on SLAF sequencing
Gastrodia elata BI. is an important cultivated medicinal plant in China. To analyze the genetic diversity and evolutionary relationship of the germplasm resources of G. elata, SNP locus and SNP ty** of G. elata
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Article
Full-Length Transcript Sequencing and Comparative Transcriptomic Analysis of Paeonia lactiflora Reveals Tissue- and Ecotype-Specific Expression of Paeoniflorin Biosynthesis-Related Genes
Paeonia lactiflora Pall., a Paeoniaceae family member, is a herbaceous perennial flower of significant horticultural importance. It holds prominence in traditional Chinese medicine due to its therapeutic applicat...
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Article
Characterization and Functional Explorations of O-glycosylation Enzymes SECRET AGENT and SPINDLY in Pyrus bretschneideri
O-linked β-N-acetylglucosamine (O-GlcNAc) modification, a specific and ubiquitous post-translational modification of proteins, has been verified to play an irreplaceable role in diverse biological processes. O-Gl...
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Article
Genome-wide assessment of genetic diversity and population structure of Platostoma palustre in southern China and implications for conservation and breeding strategies
Platostoma palustre (syn Mesona chinensis) (Lamiaceae) is an herbal plant used in traditional medicine and as food. During domestication over centuries, the plants for cultivation were propagated vegetatively by ...
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Article
Effects of 5-aminolevulinic Acid on the Bioactive Compounds and Seedling Growth of Oilseed Rape (Brassica napus L.)
Application of 5-Aminolevulinic acid (ALA) to plants not only affects their growth, but also influences the nutritional values of plant organs. Oilseed rape (Brassica napus L.) is an important source of vegetable...
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Article
Stable expression and heredity of alien Glu-1Ssh in wheat-Aegilops sharonensis hybrid progenies
In our previous study, we have isolated novel HMW-GSs with unusually large molecular weight from Aegilops sharonensis; and to introduce this novel HMW-GSs variant into common wheat, we produced a number of amphip...
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Article
Identification and Functional Characterization of R3 MYB Transcription Factor Genes in Soybean
In Arabidopsis, trichome formation is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex, which can activate the expression of GLABRA2 (GL2) and R3 MYB genes. GL2 is required for trichome format...
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Article
The rice YGL gene encoding an Mg2+-chelatase ChlD subunit is affected by temperature for chlorophyll biosynthesis
Leaf color-related mutants are important materials to study the chlorophyll biosynthesis and chloroplast development. Here, we isolated and characterized a thermosensitive yellow-green leaf mutant, ygl, through m...
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Article
Identification of a novel cytoplasmic male sterile line M2BS induced by partial-length HcPDIL5-2a transformation in rice (Oryza sativa L.)
A cytoplasmic male sterile line (designated as M2BS) was obtained from an indica rice maintainer M2B induced by partial-length HcPDIL5-2a (Hibiscus cannabinus protein disulfide isomerase-like) transformation. The...
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Article
Three P5CS genes including a novel one from Lilium regale play distinct roles in osmotic, drought and salt stress tolerance
Proline accumulations in abiotically stressed plants is generally considered to benefit their stress tolerance. The Δ1-Pyrroline-5-carboxylate synthetase (P5CS) gene family, which encodes the rate-limiting enzyme...
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Article
Characterization of the waxy gene in diploid Triticum L. and Aegilops L. species and its geographic distribution
Waxy gene plays a key role for amylose synthesis in wheat seeds. The study evaluated the Waxy gene variability in 59 accessions of six diploid Triticum and Aegilops species. The percentages of variable sites, sin...
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Article
Genetic analysis of glume hairiness (Hg) gene in bread wheat (Triticum aestivum L.)
Hairiness is a common character in planta. Glume hairiness (Hg) is present in the full spectrum of bread wheat and its close relatives and was mainly studied in phenotypic diversity, species evolution, and map...
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Article
Soybean GmAOC3 promotes plant resistance to the common cutworm by increasing the expression of genes involved in resistance and volatile substance emission in transgenic tobaccos
The evaluation and use of endogenous soybean genes is an effective strategy to minimize the yield losses caused by insects. Allene oxide cyclase (AOC) catalyzes the most important step in the biosynthesis of j...
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Article
Coordination of AtHKT1;1 and AtSOS1 facilitates Na+ and K+ homeostasis in Arabidopsis thaliana under salt stress
Reducing Na+ accumulation and maintaining K+ stability in plant is one of the key strategies for improving salt tolerance. AtHKT1;1 and AtSOS1 are not only the salt tolerance determinants themselves, but also med...
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Article
Amphidiploids between tetraploid wheat and Aegilops sharonensis Eig exhibit variations in high-molecular-weight glutenin subunits
Wide crossing is a useful method for transferring traits of interest from wild Triticeae species to wheat. In this study, Aegilops sharonensis was successfully hybridized to three tetraploid wheat...
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Article
Improvement of Sclerotinia sclerotiorum resistance in Brassica napus by using B. oleracea
Sclerotinia stem rot is one of the most serious diseases in rapeseed (Brassica napus) due to the lack of resistance sources. A high level of resistance was reported in Brassica oleracea cytodeme,...
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Article
Proteomics analysis of rice proteins up-regulated in response to bacterial leaf streak disease
Bacterial leaf streak (BLS), caused by the pathogen Xanthomonas campestris pv. Oryzicola, is a major rice disease in tropical and subtropical regions of Asia. Rice proteins responsive to BLS are still not well ch...
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Article
Overexpression of OsVP1 and OsNHX1 Increases Tolerance to Drought and Salinity in Rice
Drought and salinity are major abiotic stresses affecting rice production. To improve plant tolerance to salinity and drought, we overexpressed rice Na+/H+ exchangers (OsNHX1) and H+-pyrophosphatase in tonoplasts...
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Article
Isolation and Characterization of a Putative Vacuolar Na+/H+ Antiporter Gene from Zoysia japonica L.
A Na+/H+ antiporter gene from Zoysia japonica (ZjNHX1) which is a member of plant NHX-genes family was cloned by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE...