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  1. Article

    Open Access

    Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene

    Genetic code expansion enables the incorporation of non-canonical amino acids (ncAAs) into expressed proteins. ncAAs are usually encoded by a stop codon that is decoded by an exogenous orthogonal aminoacyl tRN...

    Noa Aloush, Tomer Schvartz, Andres I. König, Sarit Cohen in Scientific Reports (2018)

  2. Article

    Open Access

    VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis

    The hexameric AAA ATPase VPS4 facilitates ESCRT III filament disassembly on diverse intracellular membranes. ESCRT III components and VPS4 have been localized to the ciliary transition zone and spindle poles a...

    Carolyn Ott, Dikla Nachmias, Shai Adar, Michal Jarnik in Scientific Reports (2018)

  3. Article

    Open Access

    Resolving new ultrastructural features of cytokinetic abscission with soft-X-ray cryo-tomography

    Mammalian cytokinetic abscission is mediated by the ESCRT membrane fission machinery. While much has been clarified on the topology and kinetics of abscission through high-resolution microscopy, key questions ...

    Shachar Sherman, David Kirchenbuechler, Dikla Nachmias, Adi Tamir in Scientific Reports (2016)