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Transgenic Xenopus laevis embryos can be generated using φC31 integrase
Bacteriophage φC31 encodes an integrase that can mediate the insertion of extrachromosomal DNA into genomic DNA. Here we show that the coinjection of mRNA encoding φC31 integrase with plasmid DNA encoding the ...
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Article
Using phiC31 integrase to make transgenic Xenopus laevis embryos
Bacteriophage phiC31 produces the enzyme integrase that allows the insertion of the phage genome into its bacterial host. This enzyme recognizes a specific DNA sequence in the phage (attP) and a different sequenc...