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    The Length Limit of 5′ Nucleotide Additions to PCR Primers

    The addition of nucleotides to polymerase chain reaction (PCR) primers has become a widely used technique to facilitate the cloning of PCR products. A long fragment mismatched with template, such as an epitope...

    Chang-En Tian, Tao-Wen Hong, Yu-** Zhou… in National Academy Science Letters (2018)