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Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
Many viral proteins influence the cellular pathways that control cell proliferation and cell death. Some viral proteins trigger apoptotic cell death,... -
Analysis of the Regulation of Viral Transcription
Despite the small genomes and number of genes of papillomaviruses, regulation of their transcription is very complex and governed by numerous... -
Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies
The generation of cell strains and established cell lines from human papillomavirus (HPV)-infected cervical biopsies and ano-genital warts is best... -
Identification of New Papillomavirus Types
The identification of papillomavirus DNA sequences in tissue samples using polymerase chain reaction (PCR) amplification, has led to the association... -
Codon Optimization of Papillomavirus Genes
Early and late genes of human and animal papillomaviruses show a codon composition seemingly unfavorable for expression in mammalian cells. It... -
Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
Improvements in methodologies to recapitulate and study particular biological functions of the papillomavirus life cycle have led to great advances... -
Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
The genomes of human and animal papillomaviruses amplify in keratinocytes undergoing terminal squamous differentiation. Two approaches have been... -
The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
Animal models are essential to study the pathogenesis of papillomavirus infection and develop strategies for treatment and prevention. This review... -
Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
Human papillomaviruses (HPVs) are known to be etiological agents of cervical cancer and have been found in 99.7% of women with high-grade (HG)... -
The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
The permissive propagation of papillomaviruses outside their natural hosts has not been possible, which is an important restriction to the study of... -
Histochemical Analysis of Cutaneous HPV-Associated Lesions
Hematoxylin and eosin (H&E) staining of cutaneous warts is presented to illustrate the practical methods utilized for histochemical analysis of... -
Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture
The organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus... -
Analysis of Regulatory Motifs Within HPV Transcripts
Papillomavirus late gene expression is highly dependent on host epithelial cell differentiation, such that capsid proteins are produced only in... -
Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
The treatment of cervical intraepithelial neoplasia (CIN) depends on the evaluation of CIN grade. The grading of CIN is however problematic, as... -
Histological Analysis of Cervical Intraepithelial Neoplasia
A wide interobserver variation is seen even among competent histopathologists in the routine diagnosis of cervical intraepithelial neoplasia (CIN).... -
In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
The high-risk mucosal human papillomavirus E6 proteins were the first viral proteins that were shown to use the ubiquitin proteasome pathway for the... -
HPV DNA Detection and Ty** in Inapparent Cutaneous Infections and Premalignant Lesions
Epidemiological studies, which address the role of human papillomavirus (HPV) in the pathogenesis of (pre)malignant cutaneous lesions, focus on the... -
Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers
Most cervical cancers are preventable when the precursor lesions are detected in time. Human papilloma viruses (HPVs) are the main risk factors for... -
Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
Human papillomavirus (HPV) productive infection has been long considered to be restricted to the squamous epithelium. However, we have demonstrated... -
Analysis of E7/Rb Associations
The product of the early gene E7 is one of the major transforming proteins of human papillomaviruses (HPVs). It exerts its activity by associating...