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Induction and Regulation of Inflammatory Bowel Disease in Immunodeficient Mice by Distinct CD4+ T-Cell Subsets
Although the etiology of human inflammatory bowel disease (IBD) has not yet been completely defined, the current prevailing hypothesis is that it is...
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Inducing Mixed Chimerism and Transplantation Tolerance Through Allogeneic Bone Marrow Transplantation With Costimulation Blockade
Induction of mixed chimerism (i.e., coexistence of donor and recipient hematopoietic cells) through transplantation of allogeneic donor bone marrow...
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Induction of Tolerance by Adoptive Transfer of Treg Cells
Naturally arising CD4+CD25+ regulatory T (Treg) cells can be exploited to establish immunologic tolerance to allogeneic transplants. In vivo exposure...
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Generation of Immunocompetent T Cells from Embryonic Stem Cells
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted...
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Genetic Modification of Dendritic Cells Through the Directed Differentiation of Embryonic Stem Cells
Recent years have witnessed a progressive acceptance of the dual role played by dendritic cells (DC) in the initiation of immune responses and their...
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Antigen-Based Therapy and Immune-Regulation in Experimental Autoimmune Encephalomyelitis
Experimental autoimmune encephalomyelitis is a long-established mouse model of multiple sclerosis. The requirements for autoreactive T-cell...
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Modulation of the Immune Response Using Dendritic Cell-Derived Exosomes
Initial studies in our laboratory were focused on the use of dendritic cells (DC) genetically modified to express Th2-derived cytokines (i.e.,...
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Thymic Involution
The thymus contributes to the regulation of tolerance and the prevention of autoimmunity at many levels. First, auto-reactive CD4+ and CD8+ T cells...
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Gene Expression Profiling of Dendritic Cells by Microarray
The immune system of vertebrate animals has evolved to respond to different types of perturbations (invading pathogens, stress signals), limiting...
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Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles
Analysis of immune reconstitution is of major importance in clinical settings such as following bone marrow transplantation or during anti-retroviral...
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Induction of Dominant Tolerance Using Monoclonal Antibodies
Monoclonal antibodies (MAb) have been shown to be effective in inducing immune tolerance in transplantation and autoimmunity. Several different MAb...
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Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells
Regulatory T cells play a central role in controlling homeostasis, and in inducing and maintaining tolerance to both foreign and self-antigens....
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Frontiers of Immunological Tolerance
Herein, we succinctly review mechanisms underlying self-tolerance and the roles of dendritic leukocytes (DCs) in T-cell tolerance to self and foreign...
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Balancing Tolerance and Immunity
The interaction between dendritic cells and T cells is crucial for the regulation of immunological tolerance and immunity. Although our understanding...
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Generation of a Tissue-Engineered Thymic Organoid
The thymic microenvironment provides essential support for the generation of a functional and diverse population of human T cells. In particular, the...
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Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node
Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a...
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Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines
The major histocompatibility complex (MHC)-restricted presentation of self-peptides, generated from tissue-specific antigens, by thymic epithelial...
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Identification of HPV Variants
The vast majority of anogenital carcinomas are caused by high-risk human papillomaviruses (HPVs), and among Western nations HPV-16 is usually the...
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Analysis of HPV Transcription by RPA
Human papillomavirus (HPV) transcription is a complex process using multiple promoters, splices, and polyadenylation sites to create polycistronic...
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Analysis of Adeno-Associated Virus and HPV Interaction
It is slowly becoming accepted that adeno-associated virus (AAV) is another significant factor involved in cervical carcinogenesis. However, unlike...